In Silico Detection of Integrons and Their Relationship with Resistance Phenotype of Salmonella Isolates from a Brazilian Pork Production Chain.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Foodborne pathogens and disease Pub Date : 2024-06-01 Epub Date: 2024-04-03 DOI:10.1089/fpd.2023.0118

Rafaela da Silva Rodrigues, Natália Ferreira de Araujo, Cibeli Viana, Ricardo Seiti Yamatogi, Luís Augusto Nero

{"title":"In Silico Detection of Integrons and Their Relationship with Resistance Phenotype of Salmonella Isolates from a Brazilian Pork Production Chain.","authors":"Rafaela da Silva Rodrigues, Natália Ferreira de Araujo, Cibeli Viana, Ricardo Seiti Yamatogi, Luís Augusto Nero","doi":"10.1089/fpd.2023.0118","DOIUrl":null,"url":null,"abstract":"The pork production chain is an important reservoir of antimicrobial resistant bacteria. This study identified and characterized integrons in Salmonella isolates from a Brazilian pork production chain and associate them with their antibiotic resistance pattern. A total of 41 whole-genome sequencing data of nontyphoidal Salmonella were analyzed using PlasmidSPAdes and IntegronFinder software. Nine isolates (21.9%) had some integrons identified (complete and/or incomplete). Six complete class 1 integrons were found, with streptomycin resistance genes (aadA1, aadA2) alone or downstream of a trimethoprim resistance gene (dfrA1, dfrA12), and some also containing resistance genes for sulfonamides (sul1, sul3) and chloramphenicol (cmlA1). Class 2 integron was detected in only one isolate, containing dfrA1-sat2-aadA1 gene cassettes. Five isolates harbored CALINs-clusters attC but lacking integrases-with antimicrobial resistance genes typically found in integron structures. In all, integrons were observed among four serotypes: Derby, Bredeney, Panama, and monophasic var. Typhimurium I 4,[5],12:i:-. The association of integrons with antibiotic resistance phenotype showed that these elements were predominantly identified in multidrug resistance isolates, and six of the seven gentamicin-resistant isolates had integrons. So, surveillance of integrons in Salmonella should be performed to identify the potential for the spread of antimicrobial resistance genes among bacteria.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Foodborne pathogens and disease","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1089/fpd.2023.0118","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/4/3 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The pork production chain is an important reservoir of antimicrobial resistant bacteria. This study identified and characterized integrons in Salmonella isolates from a Brazilian pork production chain and associate them with their antibiotic resistance pattern. A total of 41 whole-genome sequencing data of nontyphoidal Salmonella were analyzed using PlasmidSPAdes and IntegronFinder software. Nine isolates (21.9%) had some integrons identified (complete and/or incomplete). Six complete class 1 integrons were found, with streptomycin resistance genes (aadA1, aadA2) alone or downstream of a trimethoprim resistance gene (dfrA1, dfrA12), and some also containing resistance genes for sulfonamides (sul1, sul3) and chloramphenicol (cmlA1). Class 2 integron was detected in only one isolate, containing dfrA1-sat2-aadA1 gene cassettes. Five isolates harbored CALINs-clusters attC but lacking integrases-with antimicrobial resistance genes typically found in integron structures. In all, integrons were observed among four serotypes: Derby, Bredeney, Panama, and monophasic var. Typhimurium I 4,[5],12:i:-. The association of integrons with antibiotic resistance phenotype showed that these elements were predominantly identified in multidrug resistance isolates, and six of the seven gentamicin-resistant isolates had integrons. So, surveillance of integrons in Salmonella should be performed to identify the potential for the spread of antimicrobial resistance genes among bacteria.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

巴西猪肉生产链中沙门氏菌分离物的整合子及其与抗性表型关系的硅学检测

猪肉生产链是耐抗生素细菌的重要贮藏地。本研究发现并鉴定了巴西猪肉生产链中沙门氏菌分离物中的整合子，并将其与抗生素耐药性模式联系起来。研究人员使用 PlasmidSPAdes 和 IntegronFinder 软件分析了 41 个非伤寒沙门氏菌的全基因组测序数据。九个分离株（21.9%）的一些整合子被鉴定出来（完整和/或不完整）。发现了 6 个完整的 1 级整合子，其中有链霉素抗性基因（aadA1、aadA2），也有三甲氧苄青霉素抗性基因（dfrA1、dfrA12）的下游基因，有些还含有磺胺类药物（sul1、sul3）和氯霉素（cmlA1）的抗性基因。只有一个分离株中检测到 2 级整合子，含有 dfrA1-sat2-aadA1 基因盒。5 个分离物含有 CALINs-簇 attC，但缺乏整合酶，其抗菌抗性基因通常存在于整合子结构中。总之，在四个血清型中观察到了整合子：Derby、Bredeney、巴拿马和单相变种 Typhimurium I 4、[5]、12:i:-。整合子与抗生素耐药性表型的关联表明，这些元件主要出现在耐多药的分离物中，7 个耐庆大霉素的分离物中有 6 个含有整合子。因此，应对沙门氏菌中的整合子进行监测，以确定抗菌素耐药基因在细菌中传播的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.