{"title":"Identification of oral bacteria as a new forensic tool for saliva detection","authors":"Gili Betser-Cohen , Lina Mayuoni-Kirsehenbaum , Khaled Zubeidat , Yael Horev , Luba Eli Berchoer , Adiel Cohen , Doron Aframian , Merav Amiel , Avi-Hai Hovav","doi":"10.1016/j.forsciint.2024.112112","DOIUrl":null,"url":null,"abstract":"<div><p>Body fluid detection is an important component in the toolbox of forensic scientists, with saliva playing a particularly critical role in forensic evidence. Given that each body fluid possesses a distinct microbiome, the identification of body fluid based on specific representatives of the microbiota presents an appealing approach for forensic applications. In this study, we have developed a real-time polymerase chain reaction (RT-PCR)-based method for the precise identification of saliva, focusing on three bacteria highly associated with saliva but not with other tested body fluids -<em>Porphyromonas gingivalis</em>, <em>Fusobacterium nucleatum</em>, and <em>Streptococcus salivarius</em>. The inclusion of these three bacterial species enhances the accuracy of detection and reinforces validation. Notably, specific identification of saliva was achievable even at low concentrations where Phadebas, a commonly used method for saliva detection, proved ineffective. Importantly, bacteria-based saliva detection utilizes DNA generated for small tandem repeats (STR) profiling, facilitating seamless integration into forensic laboratories and optimizing DNA sample utilization. This study collectively proposes an effective bacterial DNA-based approach for saliva identification, demonstrating promising potential for forensic applications.</p></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Forensic science international","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0379073824001932","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, LEGAL","Score":null,"Total":0}
引用次数: 0
Abstract
Body fluid detection is an important component in the toolbox of forensic scientists, with saliva playing a particularly critical role in forensic evidence. Given that each body fluid possesses a distinct microbiome, the identification of body fluid based on specific representatives of the microbiota presents an appealing approach for forensic applications. In this study, we have developed a real-time polymerase chain reaction (RT-PCR)-based method for the precise identification of saliva, focusing on three bacteria highly associated with saliva but not with other tested body fluids -Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus salivarius. The inclusion of these three bacterial species enhances the accuracy of detection and reinforces validation. Notably, specific identification of saliva was achievable even at low concentrations where Phadebas, a commonly used method for saliva detection, proved ineffective. Importantly, bacteria-based saliva detection utilizes DNA generated for small tandem repeats (STR) profiling, facilitating seamless integration into forensic laboratories and optimizing DNA sample utilization. This study collectively proposes an effective bacterial DNA-based approach for saliva identification, demonstrating promising potential for forensic applications.
体液检测是法医科学家工具箱中的重要组成部分,其中唾液在法医证据中的作用尤为关键。鉴于每种体液都具有独特的微生物群,根据微生物群的特定代表对体液进行鉴定是一种具有吸引力的法医应用方法。在这项研究中,我们开发了一种基于实时聚合酶链式反应(RT-PCR)的方法来精确鉴定唾液,重点是与唾液高度相关的三种细菌,但与其他测试体液无关--牙龈卟啉单胞菌、核酸镰刀菌和唾液链球菌。这三种细菌的加入提高了检测的准确性并加强了验证。值得注意的是,即使在唾液检测常用方法 Phadebas 无效的低浓度条件下,也能对唾液进行特异性鉴定。重要的是,基于细菌的唾液检测利用了小串联重复序列(STR)图谱生成的 DNA,有利于无缝集成到法医实验室,并优化 DNA 样本的利用。本研究共同提出了一种有效的基于细菌 DNA 的唾液鉴定方法,为法医应用展示了巨大的潜力。
期刊介绍:
Forensic Science International is the flagship journal in the prestigious Forensic Science International family, publishing the most innovative, cutting-edge, and influential contributions across the forensic sciences. Fields include: forensic pathology and histochemistry, chemistry, biochemistry and toxicology, biology, serology, odontology, psychiatry, anthropology, digital forensics, the physical sciences, firearms, and document examination, as well as investigations of value to public health in its broadest sense, and the important marginal area where science and medicine interact with the law.
The journal publishes:
Case Reports
Commentaries
Letters to the Editor
Original Research Papers (Regular Papers)
Rapid Communications
Review Articles
Technical Notes.