Mass spectrometric characterization of aminophospholipids containing N-(2-hydroxyethyl)glycine in kombu algae extracts

IF 1.8 3区 化学 Q4 BIOCHEMICAL RESEARCH METHODS Rapid Communications in Mass Spectrometry Pub Date : 2024-06-24 DOI:10.1002/rcm.9843
Giovanni Ventura, Mariachiara Bianco, Cosima Damiana Calvano, Giuliana Bianco, Angela Di Capua, Davide Coniglio, Ilario Losito, Tommaso R. I. Cataldi
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Abstract

Rationale

1,2-Diacyl-sn-glycero-3-phospho-O-[N-(2-hydroxyethyl)glycines] (PHEGs) are a class of rare aminophospholipids found specifically in brown algae, including kombu seaweed. Despite their potential importance in algal physiology, a comprehensive mass spectrometry (MS) characterization, useful to understand their biological behaviour, is still lacking.

Methods

To establish the structural regiochemical features of PHEGs, we employed hydrophilic interaction liquid chromatography (HILIC). Following separation, the isolated band of PHEGs was analyzed using MS techniques. This included multistage tandem MS experiments, performed in both positive and negative electrospray ionization modes at low and high resolution.

Results

By comparing MS/MS and MS3 spectra acquired in negative ion mode, the regiochemical rules for PHEG identification were established. The most abundant PHEG species in kombu seaweed, from both Laminaria ochroleuca (European Atlantic) and Laminaria longissima (Japan), was identified as PHEG 20:4/20:4. Less abundant species included PHEG 20:4/20:5 and hydroxylated forms of both PHEG 20:4/20:4 (i.e. 40:8;O) and 20:4/20:5 (40:9;O). The presence of a lyso PHEG 20:4 was consistently detected but at very low levels.

Conclusions

This study employed MS analysis to elucidate the regiochemical patterns of PHEGs in kombu seaweed. We identified PHEG 20:4/20:4 as the dominant species, along with several less abundant variants, including hydroxylated forms. These findings provide valuable insights into the potential roles and metabolism of PHEGs in brown algae, paving the way for further investigation into their biological functions.

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昆布藻类提取物中含有 N-(2-羟乙基)甘氨酸的氨基磷脂的质谱特征。
理由:1,2-二乙酰基-sn-甘油-3-磷酰-O-[N-(2-羟乙基)甘氨酸](PHEGs)是一类罕见的氨基磷脂,专门存在于包括昆布海藻在内的褐藻中。尽管 PHEGs 在藻类生理学中具有潜在的重要性,但目前仍缺乏有助于了解其生物行为的全面质谱(MS)表征:为了确定 PHEGs 的结构区域化学特征,我们采用了亲水作用液相色谱法(HILIC)。分离后,利用质谱技术对分离出的 PHEGs 条带进行分析。这包括在低分辨率和高分辨率下以正离子和负离子电喷雾电离模式进行的多级串联质谱实验:结果:通过比较在负离子模式下获得的 MS/MS 和 MS3 图谱,建立了 PHEG 识别的区域化学规则。经鉴定,昆布海藻中含量最高的 PHEG 为 PHEG 20:4/20:4,分别来自 Laminaria ochroleuca(欧洲大西洋)和 Laminaria longissima(日本)。含量较少的物种包括 PHEG 20:4/20:5 以及 PHEG 20:4/20:4 的羟基化形式(即 40:8;O)和 20:4/20:5 的羟基化形式(40:9;O)。始终检测到溶菌 PHEG 20:4,但含量很低:本研究利用质谱分析阐明了昆布海藻中 PHEG 的区域化学模式。我们发现 PHEG 20:4/20:4 是最主要的种类,此外还有几种含量较低的变体,包括羟基化形式。这些发现为了解 PHEGs 在褐藻中的潜在作用和新陈代谢提供了宝贵的见解,为进一步研究它们的生物功能铺平了道路。
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来源期刊
CiteScore
4.10
自引率
5.00%
发文量
219
审稿时长
2.6 months
期刊介绍: Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.
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