Improved productivity and dye removal performance of Trametes versicolor pellets using rice husk as a co-substrate

IF 1.7 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS Journal of microbiological methods Pub Date : 2024-06-24 DOI:10.1016/j.mimet.2024.106976

Juan Antonio Gutiérrez-Quirós , Alonso Coronado-Marchena , Diego Villegas-Solano, Sebastián Rodríguez-Saravia, Víctor Castro-Gutiérrez, Carlos E. Rodríguez-Rodríguez

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Abstract

Pellet production represents a critical step for several processes requiring fungal biomass, nevertheless, its optimization is seldom reported. The use of finely ground rice husk as a microcarrier and co-substrate permitted a marked increase (≈ 2.7×) in the productivity of fungal pellet production using Trametes versicolor compared to traditional production methods. The pellets show similar structure and smaller size compared to typical sole-mycelium pellets, as well as comparable laccase activity. The efficiency of the pellets for biodegradation was confirmed by the removal of the crystal violet dye, achieving significantly faster decolorization rates compared to the traditionally produced pellets. The use of these pellets during the continuous treatment of the dye in a stirred tank bioreactor resulted in 97% decolorization operating at a hydraulic residence time of 4.5 d.

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使用稻壳作为共基质提高多色曲霉颗粒的生产率和染料去除性能。

颗粒生产是一些需要真菌生物质的工艺中的关键步骤，但其优化却鲜有报道。与传统生产系统相比，使用磨细的稻壳作为微载体和辅助底物，可以显著提高（≈ 2.7 倍）使用多色真菌生产真菌颗粒的生产率。与典型的单菌丝颗粒相比，这种颗粒结构相似，体积更小，漆酶活性也相当。这种颗粒的生物降解效率通过对水晶紫染料的去除得到了证实，与传统生产的颗粒相比，其去除率要快得多。在搅拌槽生物反应器中连续处理染料时使用这些颗粒，在水力停留时间为 4.5 d 的情况下，脱色率达到 97%。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.