Machine Learning for Single-Molecule Localization Microscopy: From Data Analysis to Quantification

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Analytical Chemistry Pub Date : 2024-06-30 DOI:10.1021/acs.analchem.3c05857
Jianli Liu, Yumian Li, Tailong Chen, Fa Zhang* and Fan Xu*, 
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Abstract

Single-molecule localization microscopy (SMLM) is a versatile tool for realizing nanoscale imaging with visible light and providing unprecedented opportunities to observe bioprocesses. The integration of machine learning with SMLM enhances data analysis by improving efficiency and accuracy. This tutorial aims to provide a comprehensive overview of the data analysis process and theoretical aspects of SMLM, while also highlighting the typical applications of machine learning in this field. By leveraging advanced analytical techniques, SMLM is becoming a powerful quantitative analysis tool for biological research.

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单分子定位显微镜的机器学习:从数据分析到量化。
单分子定位显微镜(SMLM)是利用可见光实现纳米级成像的多功能工具,为观察生物过程提供了前所未有的机会。机器学习与单分子定位显微镜的整合提高了数据分析的效率和准确性。本教程旨在全面介绍 SMLM 的数据分析过程和理论方面,同时强调机器学习在该领域的典型应用。通过利用先进的分析技术,SMLM 正在成为生物研究领域强大的定量分析工具。
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来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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