Determination of colostrum quality using Brix refractometer in sheep.

IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Acta veterinaria Hungarica Pub Date : 2024-06-27 Print Date: 2024-07-03 DOI:10.1556/004.2024.00951
Meryem Sarica, Uğur Aydoğdu
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Abstract

In this study, we aimed to determine the quality of colostrum in sheep by using Brix refractometer. The research included 100 sheep of Merino X Kivircik crossbred. From each, we collected 15 mL of colostrum samples in falcon tubes within the first 8 h after delivery. Mean colostral IgG level of sheep was 156.68 ± 7.23 g L-1, optical and digital Brix refractometer values (%) were determined as 27.43 ± 0.53 and 27.69 ± 0.60, respectively. Ewes carrying twin lambs produced significantly higher quality colostrum than those carrying single lambs. However, parity did not affect the colostrum quality. Optical and digital Brix values were correlated with gold standard radial immunodiffusion (RID) colostral IgG level (r = 0.70 and r = 0.64, respectively). Also, optical and digital Brix refractometers were found to be highly correlated (r = 0.98, P < 0.001). While the optimal Brix value was 22% for the 50, 60 and 70 g L-1 IgG threshold values (by means of RID as the potential good quality threshold value for ewe colostrum quality), this value was 23% for 80 g L-1. We can conclude that Brix refractometers is a valuable tool for determining ewe colostrum quality. A cut point of 22% Brix for defining good quality colostrum in ewes was most appropriate for our data.

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使用 Brix 折射仪测定绵羊初乳的质量。
在这项研究中,我们的目的是使用 Brix 折光仪测定绵羊初乳的质量。研究对象包括 100 只美利奴 X Kivircik 杂交绵羊。我们在每只绵羊产后 8 小时内用猎鹰管采集了 15 mL 的初乳样本。绵羊初乳 IgG 的平均水平为 156.68 ± 7.23 g L-1,光学和数字 Brix 折射仪测定值(%)分别为 27.43 ± 0.53 和 27.69 ± 0.60。怀有双羔的母羊生产的初乳质量明显高于怀有单羔的母羊。然而,胎次并不影响初乳质量。光学和数字 Brix 值与金标准径向免疫扩散(RID)初乳 IgG 水平相关(r = 0.70 和 r = 0.64)。此外,还发现光学和数字 Brix 折射仪高度相关(r = 0.98,P < 0.001)。50、60 和 70 g L-1 IgG 临界值的最佳 Brix 值为 22%(通过 RID 作为母羊初乳质量的潜在优质临界值),而 80 g L-1 的最佳 Brix 值为 23%。我们可以得出结论,Brix 折射仪是确定母初乳质量的重要工具。根据我们的数据,Brix 为 22% 的临界值最适合用于定义母羊优质初乳。
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来源期刊
Acta veterinaria Hungarica
Acta veterinaria Hungarica 农林科学-兽医学
CiteScore
1.80
自引率
0.00%
发文量
39
审稿时长
>36 weeks
期刊介绍: Acta Veterinaria Hungarica publishes original research papers presenting new scientific results of international interest, and to a limited extent also review articles and clinical case reports, on veterinary physiology (physiological chemistry and metabolism), veterinary microbiology (bacteriology, virology, immunology, molecular biology), on the infectious diseases of domestic animals, on veterinary parasitology, pathology, clinical veterinary science and reproduction.
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