Influence of different penetrating cryoprotectants and egg yolk level on the post-thaw quality of sperm.

IF 2.4 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animal Bioscience Pub Date : 2024-06-25 DOI:10.5713/ab.24.0167

Liuming Zhang, Caiyu Jiang, Xuyang Wang, Tariq Sohail, Yuxuan Sun, Jian Wang, Xiaomei Sun, Yongjun Li

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Abstract

Objective: The Hu sheep is a renowned breed known for its high reproductive rate. It is in estrus all year round, and its breeding population is gradually expanding. However, the current techniques for cryopreserving semen have limited effectiveness, which hinders the continuous development of this species. The purpose of this study is to explore the effects of different penetrating cryoprotectants (CPAs) and egg yolk (EY) concentrations on the cryopreservation of Hu ram semen to determine the most effective combination.

Methods: In this study, the effects of glycerol (GLY), ethylene glycol (EG), dimethylacetamide (DMA), dimethyl sulfoxide (DMSO), different proportions of GLY and EG, EY on sperm quality after thawing were investigated by detecting sperm total motility (TM), progressive motility (PM), straight-line velocity (VSL), curvilinear velocity (VCL), average path velocity (VAP), amplitude of lateral head displacement (ALH), wobble movement coefficient (WOB), average motion degree (MAD), functional integrity (plasma membrane integrity, acrosome integrity) and reactive oxygen species (ROS) level.

Results: When GLY and EG were added together, compared to other concentration groups, 6% GLY significantly (p < 0.05) increased TM, PM, plasma membrane integrity, and acrosome integrity of thawed sperm. Additionally, it significantly (p < 0.05) decreased the ROS level of sperm. In this study, the TM, PM and membrane integrity of the 6% EG were significantly (p < 0.05) higher than those of the control, 1% GLY+5% EG and 6% GLY+6% EG groups. Compared to other concentration groups, 20% EY significantly (p < 0.05) improved the TM, PM, and plasma membrane integrity of thawed sperm. However, the integrity of the acrosome increased with the higher concentration of EY.

Conclusion: In conclusion, the post-thawed Hu ram semen diluted with a diluent containing 6% GLY and 20% EY exhibited higher quality compared to the other groups.

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不同渗透性冷冻保护剂和卵黄水平对精子解冻后质量的影响

目的：胡羊是一个以繁殖率高而闻名的品种。它常年发情，繁殖数量也在逐渐扩大。然而，目前的精液冷冻保存技术效果有限，阻碍了该品种的持续发展。本研究旨在探讨不同渗透性冷冻保护剂（CPAs）和蛋黄（EY）浓度对胡公羊精液冷冻保存的影响，以确定最有效的组合：本研究通过检测精子的总运动能力（TM）、渐进运动能力（PM）、直线速度（VSL）、曲线速度（VSL）和精子的总运动能力（TM）、渐进运动能力（PM）、直线速度（VSL）、曲线速度（VSL）和精子的总运动能力（TM）来研究甘油（GLY）、乙二醇（EG）、二甲基乙酰胺（DMA）、二甲基亚砜（DMSO）、不同比例的甘油（GLY）和乙二醇（EG）、蛋黄（EY）对解冻后精子质量的影响、曲速（VCL）、平均路径速度（VAP）、头部侧移幅度（ALH）、摇摆运动系数（WOB）、平均运动度（MAD）、功能完整性（质膜完整性、顶体完整性）和活性氧（ROS）水平。结果当 GLY 和 EG 同时添加时，与其他浓度组相比，6% GLY 能显著（p < 0.05）提高解冻精子的 TM、PM、质膜完整性和顶体完整性。此外，它还能明显（p < 0.05）降低精子的 ROS 水平。在本研究中，6% EG 组的 TM、PM 和膜完整性明显高于对照组、1% GLY+5% EG 组和 6% GLY+6% EG 组（p < 0.05）。与其他浓度组相比，20% EY能明显改善解冻精子的TM、PM和质膜完整性（p < 0.05）。然而，顶体的完整性随着 EY 浓度的升高而增加：总之，与其他组相比，用含有 6% GLY 和 20% EY 的稀释液稀释的解冻后胡公羊精液质量更高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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