The contrasting roles of co-solvents in protein formulations and food products

IF 3.3 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biophysical chemistry Pub Date : 2024-06-17 DOI:10.1016/j.bpc.2024.107282
Tsutomu Arakawa , Yui Tomioka , Teruo Akuta , Kentaro Shiraki
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Abstract

Protein aggregation is a major hurdle in developing biopharmaceuticals, in particular protein formulation area, but plays a pivotal role in food products. Co-solvents are used to suppress protein aggregation in pharmaceutical proteins. On the contrary, aggregation is encouraged in the process of food product making. Thus, it is expected that co-solvents play a contrasting role in biopharmaceutical formulation and food products. Here, we show several examples that utilize co-solvents, e.g., salting-out salts, sugars, polyols and divalent cations in promoting protein-protein interactions. The mechanisms of co-solvent effects on protein aggregation and solubility have been studied on aqueous protein solution and applied to develop pharmaceutical formulation based on the acquired scientific knowledge. On the contrary, co-solvents have been used in food industries based on empirical basis. Here, we will review the mechanisms of co-solvent effects on protein-protein interactions that can be applied to both pharmaceutical and food industries and hope to convey knowledge acquired through research on co-solvent interactions in aqueous protein solution and formulation to those involved in food science and provide those involved in protein solution research with the observations on aggregation behavior of food proteins.

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蛋白质配方和食品中助溶剂的不同作用。
蛋白质聚集是开发生物制药,尤其是蛋白质配方领域的一大障碍,但在食品中也起着举足轻重的作用。共溶剂用于抑制制药蛋白质中的蛋白质聚集。相反,在食品制作过程中,蛋白质的聚集却会被促进。因此,预计助溶剂在生物制药配方和食品中发挥着截然不同的作用。在此,我们将展示几个利用助溶剂(如盐类、糖类、多元醇和二价阳离子)促进蛋白质-蛋白质相互作用的例子。在蛋白质水溶液中研究了助溶剂对蛋白质聚集和溶解度的影响机制,并根据所获得的科学知识将其应用于药物制剂的开发。相反,助溶剂在食品工业中的应用是基于经验基础。在此,我们将综述可应用于制药和食品行业的共溶剂对蛋白质-蛋白质相互作用的影响机制,并希望将通过研究共溶剂在蛋白质水溶液和配方中的相互作用所获得的知识传达给食品科学相关人员,并为蛋白质溶液研究相关人员提供有关食品蛋白质聚集行为的观察结果。
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来源期刊
Biophysical chemistry
Biophysical chemistry 生物-生化与分子生物学
CiteScore
6.10
自引率
10.50%
发文量
121
审稿时长
20 days
期刊介绍: Biophysical Chemistry publishes original work and reviews in the areas of chemistry and physics directly impacting biological phenomena. Quantitative analysis of the properties of biological macromolecules, biologically active molecules, macromolecular assemblies and cell components in terms of kinetics, thermodynamics, spatio-temporal organization, NMR and X-ray structural biology, as well as single-molecule detection represent a major focus of the journal. Theoretical and computational treatments of biomacromolecular systems, macromolecular interactions, regulatory control and systems biology are also of interest to the journal.
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