Elevated VEGF-A Levels in the Aqueous Humor of Patients With Primary Open Angle Glaucoma.

IF 1.8 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL In vivo Pub Date : 2024-07-01 DOI:10.21873/invivo.13642

Georgios S Dimtsas, Alexandra Ieronymaki, Klio I Chatzistefanou, Gerasimos Siasos, Augustinos Krassas, Marilita M Moschos

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Abstract

Background/aim: The purpose of the current study was to compare the vascular endothelial growth factor-A (VEGF-A) levels in the aqueous humor of patients with primary open angle glaucoma (POAG) and non-glaucomatous eyes and reveal any potential statistically significant correlations.

Patients and methods: This was an observational cross-sectional study. Aqueous humor samples (50-100 μl) were collected under aseptic conditions, from the anterior chamber at the start of glaucoma or cataract surgery. The levels of VEGF-A were measured using a multiplex bead-based immunoassay.

Results: Aqueous humor samples were obtained from 76 participants: 39 with POAG and 36 with age-related cataracts as controls. VEGF-A levels were significantly elevated in the POAG group (166.37±110.04 pg/ml, p=0.011) compared to the control group (119.02±49.09 pg/ml). The receiver operating characteristic (ROC) analysis showed that VEGF-A had significant prognostic ability for POAG (AUC=0.67; p=0.006). An optimal cut-off for VEGF-A was found to be 148.5 pg/ml with a sensitivity of 54%, specificity of 81.1%, positive prognostic value (PPV) of 75% and negative prognostic value (NPV) of 62.5%. Logistic regression analysis showed that after adjusting for sex and age, patients with VEGF-A higher than 148.5 pg/ml had almost 10 times greater likelihood for POAG.

Conclusion: VEGF-A is elevated in patients with POAG and can potentially have a prognostic ability for these patients.

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原发性开角型青光眼患者眼液中 VEGF-A 水平升高。

背景/目的：本研究旨在比较原发性开角型青光眼（POAG）患者和非青光眼患者眼房水中的血管内皮生长因子-A（VEGF-A）水平，并揭示任何潜在的具有统计学意义的相关性：这是一项观察性横断面研究。在无菌条件下，从青光眼或白内障手术开始时的前房采集房水样本（50-100 μl）。采用多重微珠免疫测定法测定 VEGF-A 的水平：结果：共采集了 76 名参与者的房水样本：结果：从 76 名参与者（39 名患有 POAG，36 名患有老年性白内障，作为对照组）中获得了眼房水样本。与对照组（119.02±49.09 pg/ml）相比，POAG 组 VEGF-A 水平明显升高（166.37±110.04 pg/ml，p=0.011）。接收器操作特征（ROC）分析显示，VEGF-A 对 POAG 有显著的预后能力（AUC=0.67；P=0.006）。VEGF-A的最佳临界值为148.5 pg/ml，灵敏度为54%，特异性为81.1%，阳性预后值（PPV）为75%，阴性预后值（NPV）为62.5%。逻辑回归分析表明，在对性别和年龄进行调整后，VEGF-A高于148.5 pg/ml的患者发生POAG的可能性几乎增加了10倍：结论：VEGF-A 在 POAG 患者中升高，可能对这些患者的预后产生影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

In vivo 医学-医学：研究与实验

CiteScore

4.20

自引率

4.30%

发文量

330

审稿时长

3-8 weeks

期刊介绍： IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management. The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.