Transcriptome Analysis in Patients With Muscle-invasive Bladder Cancer.

IF 1.8 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL In vivo Pub Date : 2024-07-01 DOI:10.21873/invivo.13615

Pietro Pepe, Michele Salemi, Giovanna Marchese, Maria Grazia Salluzzo, Giuseppe Lanza, Simona Marino, Francesca Schillaci, Anna Truda, Ludovica Pepe, Michele Pennisi

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Abstract

Background/aim: Bladder cancer (BC) is the most prevalent malignant tumor in the urinary tract, classified mainly into muscle-invasive BC (MIBC) and non-MIBC (NMIBC). Recent studies highlight the important role of changes in transcriptome activity in carcinogenesis, aiding in the identification of additional differentially regulated candidate genes, improving our understanding of the molecular basis of gene regulation in BC. This study aimed to evaluate the transcriptome of MIBC patients compared with normal subjects.

Materials and methods: mRNA sequencing was conducted using the Illumina NovaSeq 6000 Dx system in a case series comprising 11 subjects with MIBC and 19 healthy controls matched for age and sex. For functional analysis, the pathfindR package was utilized to comprehensively identify pathways enriched in omics data within active subnetworks.

Results: Our results demonstrated the presence of differentiated pathways, including spliceosome activity, oxidative phosphorylation, and chemical carcinogenesis due to reactive oxygen species, in MIBC patients compared with controls.

Conclusion: The identification of novel molecular pathways in MIBC patients could prove useful in defining cancer predisposition factors and exploring potential therapeutic options.

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肌浸润性膀胱癌患者的转录组分析

背景/目的：膀胱癌（BC）是泌尿系统最常见的恶性肿瘤，主要分为肌浸润性膀胱癌（MIBC）和非肌浸润性膀胱癌（NMIBC）。最近的研究强调了转录组活性变化在致癌过程中的重要作用，有助于鉴定更多受到不同调控的候选基因，从而提高我们对膀胱癌基因调控分子基础的认识。本研究旨在评估MIBC患者与正常人相比的转录组。材料与方法：使用Illumina NovaSeq 6000 Dx系统对11例MIBC患者和19例年龄、性别匹配的健康对照进行mRNA测序。在进行功能分析时，使用了 pathfindR 软件包，以在活跃的子网络中全面识别在 omics 数据中富集的通路：结果：我们的研究结果表明，与对照组相比，MIBC 患者存在分化的通路，包括剪接体活性、氧化磷酸化和活性氧导致的化学致癌：结论：在MIBC患者中发现新的分子通路有助于确定癌症易感因素和探索潜在的治疗方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

In vivo 医学-医学：研究与实验

CiteScore

4.20

自引率

4.30%

发文量

330

审稿时长

3-8 weeks

期刊介绍： IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management. The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.