Osteoinductive and regenerative potential of premixed calcium-silicate bioceramic sealers on vascular wall mesenchymal stem cells

IF 5.4 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE International endodontic journal Pub Date : 2024-06-29 DOI:10.1111/iej.14098

Chiara Bernardini, Fausto Zamparini, Carlo Prati, Roberta Salaroli, Andrea Spinelli, Augusta Zannoni, Monica Forni, Maria Giovanna Gandolfi

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Abstract

Aim

The osteogenic potential of new premixed calcium-silicate-containing bioceramic sealers (Ca-Si sealers) was tested with porcine vascular wall-mesenchymal stem cells (pVW-MSCs).

Methodology

Two Ca-Si-containing sealers: Ceraseal (MetaBiomed, Cheong-si, South Korea) and AH Plus Bioceramic (Maruchi, Wonju-si, South Korea), and an epoxy resin sealer (AH Plus; Dentsply, Konstanz, Germany) as a control, were prepared according to the manufacturers' indications. All samples were allowed to set for 100% of their setting time in a sterile humid cabinet at 37°C and 95% relative humidity. pVW-MSC seeding efficiency and osteogenic differentiation were analysed as marker of gene/protein expression for up to 12 days. Mineralization assay and immunofluorescence staining were performed and evaluated over a period of 21 days. Statistical analyses were conducted using one-way analysis of variance (p < .05). Additional samples were prepared and stored under the same conditions and inspected using an environmental scanning electron microscope equipped with an energy dispersive X-ray spectroscopy system.

Results

Significantly higher cell seeding efficiency (p < .05) was observed for both Ca-Si sealers from day 8. pVW-MSCs showed a significant shift towards the osteogenic lineage only when seeded in contact with Ca-Si sealers. Gene expression of osteopontin was upregulated significantly. Collagen I and osteocalcin were clearly expressed by cells in contact with Ca-Si sealers. Mineralization granules were observed in Alizarin red assays and confocal laser scanning microscopy analysis of both Ca-Si sealers. No gene expression or granule mineralization were observed on the epoxy resin sealer.

Conclusions

Premixed Ca-Si sealers displayed a higher potential for osteogenic activity on pVW-MSCs. Epoxy resin sealer was unable to induce any osteogenic activity. The properties of both Ca-Si sealers suggest their potential as osteoinductive platforms for vascular MSCs in periapical bone.

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预混合硅酸钙生物陶瓷密封剂对血管壁间充质干细胞的骨诱导和再生潜力。

目的：用猪血管壁-间充质干细胞（pVW-MSCs）测试新型预混合含硅酸钙生物陶瓷封闭剂（Ca-Si 封闭剂）的成骨潜力：方法：两种含 Ca-Si 的封闭剂：方法：根据制造商的说明制备两种含钙硅封闭剂：Ceraseal（MetaBiomed，韩国清溪市）和AH Plus Bioceramic（Maruchi，韩国原州市），以及作为对照的环氧树脂封闭剂（AH Plus；Dentsply，德国康斯坦茨）。所有样品均在 37°C 和 95% 相对湿度的无菌潮湿柜中静置 100%。pVW-间充质干细胞的播种效率和成骨分化作为基因/蛋白表达的标记进行了长达 12 天的分析。在 21 天内进行矿化测定和免疫荧光染色。统计分析采用单因素方差分析（P 结果）：细胞播种效率显著提高（p 结论：细胞播种效率显著提高：预混合 Ca-Si 封闭剂对 pVW 间充质干细胞具有更高的成骨活性。环氧树脂封闭剂无法诱导任何成骨活性。这两种钙硅封层的特性表明，它们有可能成为根尖周骨中血管间充质干细胞的成骨诱导平台。

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来源期刊

International endodontic journal 医学-牙科与口腔外科

CiteScore

10.20

自引率

28.00%

发文量

195

审稿时长

4-8 weeks

期刊介绍： The International Endodontic Journal is published monthly and strives to publish original articles of the highest quality to disseminate scientific and clinical knowledge; all manuscripts are subjected to peer review. Original scientific articles are published in the areas of biomedical science, applied materials science, bioengineering, epidemiology and social science relevant to endodontic disease and its management, and to the restoration of root-treated teeth. In addition, review articles, reports of clinical cases, book reviews, summaries and abstracts of scientific meetings and news items are accepted. The International Endodontic Journal is essential reading for general dental practitioners, specialist endodontists, research, scientists and dental teachers.