An in vitro antiviral evaluation of punicalagin toward influenza A virus.

IF 1.9 Q3 CHEMISTRY, MEDICINAL Avicenna Journal of Phytomedicine Pub Date : 2024-07-01 DOI:10.22038/AJP.2023.23389
Fatemeh Javadi-Farsani, Ali Karimi, Hadi Razavi Nikoo, Mohammad-Taghi Moradi, Alijan Tabarraei
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Abstract

Objective: Influenza complications are mild to serious, and can cause death in some cases. A great deal of attention has been paid in recent years to the development and use of new antiviral compounds to overcome drug resistance in certain strains of the influenza virus and treat the clinical implications. This study aimed to investigate the antiviral effect of punicalagin and its associated mechanism against influenza A (H1N1) virus in vitro.

Materials and methods: the ant-influenza activity of punicalagin was studied in Madin-Darby Canine Kidney (MDCK) cells using influenza virus A/Puerto Rico/8/34 (H1N1) (PR8) using Hemagglutinin assay (HA) and 50% tissue culture infective dose (TCID50). Then, the inhibition of haemagglutination, virucidal activity, inhibitory effect at different times, replication of viral RNA and expression of viral genes were investigated.

Results: Punicalagin could inhibit influenza virus infection with 50% inhibitory concentration (IC50) of 3.98 μg/ml and selectivity index (SI) value of 6.1. Punicalagin decreased virus titers with an inhibitory effect on virus hemagglutination (p<0.05). Punicalagin also inhibited viral adsorption. The results of virus RNA replication and viral mRNA (NS1 and HA) expression after treatment with punicalagin showed significant suppression of viral mRNA expression but no effect on replication of viral RNA.

Conclusion: The results of the present study indicated that punicalagin was effective against influenza infection most probably via inhibition of haemagglutination activity and virus binding.

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针对甲型流感病毒的体外抗病毒评估。
目的:流感并发症由轻到重,在某些情况下可导致死亡。近年来,人们十分关注新型抗病毒化合物的开发和使用,以克服流感病毒某些毒株的耐药性,并治疗其临床影响。本研究旨在体外研究Punicalagin对甲型H1N1流感病毒的抗病毒作用及其相关机制。材料和方法:使用流感病毒A/Puerto Rico/8/34 (H1N1) (PR8),采用血凝素试验(HA)和50%组织培养感染剂量(TCID50),在Madin-Darby犬肾(MDCK)细胞中研究Punicalagin的抗流感活性。然后,研究了血凝抑制、杀病毒活性、不同时间的抑制效果、病毒 RNA 的复制和病毒基因的表达:结果:Punicalagin 能抑制流感病毒感染,其 50%抑制浓度(IC50)为 3.98 μg/ml,选择性指数(SI)为 6.1。Punicalagin 可降低病毒滴度,抑制病毒血凝(pC):本研究的结果表明,Punicalagin 很可能通过抑制血凝活性和病毒结合来有效预防流感感染。
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来源期刊
Avicenna Journal of Phytomedicine
Avicenna Journal of Phytomedicine CHEMISTRY, MEDICINAL-
CiteScore
3.40
自引率
4.50%
发文量
17
审稿时长
6 weeks
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