Antimicrobial Resistance in Uropathogenic Escherichia coli Strains Isolated from Relapses from Recurrent Urinary Tract Infections.

IF 2.3 4区医学 Q3 INFECTIOUS DISEASES Microbial drug resistance Pub Date : 2024-07-01 DOI:10.1089/mdr.2023.0177

Sandra Gavilanes-Parra, Pedro Chavero-Guerra, Rigoberto Hernández-Castro, Silvia Villanueva-Recillas, Angel Manjarrez-Hernández

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Abstract

Little is known about the characteristics of uropathogenic Escherichia coli (UPEC) associated with recurrent urinary tract infections (RUTIs). The present study aimed to analyze the phenotypic antimicrobial resistance of recurrent UPEC isolates attributable to either relapse or reinfection. A total of 140 E. coli strains were isolated from 70 outpatients with RUTIs. All isolates were analyzed by random amplified polymorphic DNA-polymerase chain reaction to evaluate genetic similarity between the first and second isolates. We found that 64.2% (45/70) of outpatients had a relapse with the primary infecting E. coli strain and 35.7% (25/70) had reinfection with a new E. coli strain. Compared with reinfecting strains, relapse UPEC isolates exhibited much higher antimicrobial resistance; 89% of these isolates were multidrug-resistant and 46.6% were extended-spectrum β-lactamase producers. Our study provides evidence that RUTIs are mainly driven by the persistence of the original strain in the host (relapses) despite appropriate antibiotic treatments, and only RUTIs attributed to relapses seem to favor multidrug resistance in UPEC isolates.

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从复发性尿路感染复发病例中分离出的尿路致病性大肠埃希菌菌株的抗菌药耐药性。

人们对与复发性尿路感染（RUTI）相关的尿路致病性大肠杆菌（UPEC）的特征知之甚少。本研究旨在分析因复发或再感染导致的复发 UPEC 分离物的表型抗菌药耐药性。研究人员从 70 名患 RUTI 的门诊患者身上共分离出 140 株大肠杆菌。所有分离菌株均通过随机扩增多态 DNA 聚合酶链反应进行分析，以评估第一次和第二次分离菌株之间的遗传相似性。我们发现，64.2%（45/70）的门诊患者再次感染了原发大肠杆菌菌株，35.7%（25/70）再次感染了新的大肠杆菌菌株。与再感染菌株相比，复发的 UPEC 分离物表现出更高的抗菌药耐药性；其中 89% 的分离物具有多重耐药性，46.6% 的分离物具有广谱 β 内酰胺酶。我们的研究提供的证据表明，RUTI 主要是由宿主体内的原始菌株持续存在（复发）引起的，尽管经过适当的抗生素治疗。

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来源期刊

Microbial drug resistance 医学-传染病学

CiteScore

6.00

自引率

3.80%

发文量

118

审稿时长

6-12 weeks

期刊介绍： Microbial Drug Resistance (MDR) is an international, peer-reviewed journal that covers the global spread and threat of multi-drug resistant clones of major pathogens that are widely documented in hospitals and the scientific community. The Journal addresses the serious challenges of trying to decipher the molecular mechanisms of drug resistance. MDR provides a multidisciplinary forum for peer-reviewed original publications as well as topical reviews and special reports. MDR coverage includes: Molecular biology of resistance mechanisms Virulence genes and disease Molecular epidemiology Drug design Infection control.