Algal consortia as the flexible bio-system for wastewater treatment: Effect of different light intensities on photosynthetic performance, anti-oxidative system and biodiesel production of consortia

Abstract

Competence of microalgae consortia-1 (Chlorococcum humicola and Tetradesmus sp.) and consortia-2 (Chlorococcum humicola, Scenedesmus vacuolatus and Tetradesmus sp.) was analyzed by examining their adaptability potential in wastewater under different light intensities (20 W/m² and 40 W/m²). The results depicted highest decline in nutrients and metal concentration (40–90%) at light intensity of 40 W/m² by consortia-1 and consortia-2 treated with wastewater (50%, 100%). The result of metal remediation was further confirmed by fourier transform infrared spectroscopy (FTIR) which reflected occurrence of different functional groups in selected consortia. Moreover, light intensity of 40 W/m² induced neutral-lipid accumulation (1750 cm⁻¹) in consortia-1 as depicted in FTIR spectra. The maximum active photosystem-II reaction center (79.75%), quantum yield (26.17%) and performance index (195.8%) revealed that light intensity (40 W/m²) and wastewater augmented functioning of photosystem-II in consortia-1 than consortia-2. Further, consortia-2 appeared more sensitive to oxidative stress as the concentration of oxidative stress markers (5.73–6.39 folds) was amplified after treatment with wastewater at light intensity of 40 W/m². The prodigious tolerance potential towards different light intensities and wastewater concentration of consortia-1 may be attributed to increased activity of ascorbic acid (1.16 folds), proline (1.32 folds), cysteine (2.80 folds), superoxide dismutase (3.44 folds), catalase (2.52 folds) and glutathione reductase (1.90 folds). However, abundance of saturated fatty acid (41.34%) together with high cetane number (59.07) indicated that consortia 2 can produce excellent quality biofuel at 20 W/m² while consortia-2 at 40 W/m². Overall, consortia 1 appeared competent in terms of photosynthetic performance, remediation efficiency and antioxidant defense mechanism under 20 W/m² and 40 W/m² while consortia-2 showed quality biodiesel production at 20 W/m². Thus, algal consortia based on photosynthetic performance and defense responses along with excellent biodiesel quality under different light intensity and wastewater concentration can serve as the promising biosystem for biomass and bioenergy production.