Differential diagnostic value of simultaneous detection of CD69 and HLA-DR on host T and NK cells in QFT-TB assay for identifying active tuberculosis

IF 2.8 3区医学 Q3 IMMUNOLOGY Tuberculosis Pub Date : 2024-06-27 DOI:10.1016/j.tube.2024.102537

Yiqi Yang , Fujie Zhang , Hanlu Shi , Zhongliang Zhu , Yu Zhou , Yonglie Zhou

{"title":"Differential diagnostic value of simultaneous detection of CD69 and HLA-DR on host T and NK cells in QFT-TB assay for identifying active tuberculosis","authors":"Yiqi Yang , Fujie Zhang , Hanlu Shi , Zhongliang Zhu , Yu Zhou , Yonglie Zhou","doi":"10.1016/j.tube.2024.102537","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Interferon-gamma release assay (IGRA) for tuberculosis (TB) remains limited in its ability to discriminate between active TB (ATB) and latent TB infection (LTBI). Activation markers on host T and NK cells are currently considered to be promising markers in the diagnosis of ATB.</p></div><div><h3>Methods</h3><p>This prospective observational study enrolled 213 participants and the participants were divided into ATB, LTBI, other lung-related diseases (ORD), and health control (HC) groups. CD69 and HLA-DR on T and NK cells were detected in QFT-TB assay, and a composite scoring system (TB-Flow) was created for the diagnosis of ATB.</p></div><div><h3>Results</h3><p>The expression of activation markers (CD69 and HLA-DR) were significantly increased in ATB. HLA-DR on NK cells, CD69 on T cells, and QFT-TB in the differential diagnosis of ATB and HC were all of good diagnostic value (AUC>0.90). In addition, the TB-Flow greatly improved the efficiency of differential diagnosis between ATB and LTBI (AUC=0.90, 95%CI: 0.84–0.96), with sensitivity and specificity of 79.17 % (95%CI: 64.60%–89.04 %) and 88.68 % (95%CI: 76.28%–95.31 %).</p></div><div><h3>Conclusions</h3><p>CD69 and HLA-DR on host T and NK cells are promising markers in distinguishing different TB infection status. Our blood-based TB-Flow scoring system can distinguish ATB from LTBI with good diagnostic efficacy.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102537"},"PeriodicalIF":2.8000,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1472979224000635/pdfft?md5=dddeacf5d6042df0da651f3460859831&pid=1-s2.0-S1472979224000635-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tuberculosis","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1472979224000635","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Interferon-gamma release assay (IGRA) for tuberculosis (TB) remains limited in its ability to discriminate between active TB (ATB) and latent TB infection (LTBI). Activation markers on host T and NK cells are currently considered to be promising markers in the diagnosis of ATB.

Methods

This prospective observational study enrolled 213 participants and the participants were divided into ATB, LTBI, other lung-related diseases (ORD), and health control (HC) groups. CD69 and HLA-DR on T and NK cells were detected in QFT-TB assay, and a composite scoring system (TB-Flow) was created for the diagnosis of ATB.

Results

The expression of activation markers (CD69 and HLA-DR) were significantly increased in ATB. HLA-DR on NK cells, CD69 on T cells, and QFT-TB in the differential diagnosis of ATB and HC were all of good diagnostic value (AUC>0.90). In addition, the TB-Flow greatly improved the efficiency of differential diagnosis between ATB and LTBI (AUC=0.90, 95%CI: 0.84–0.96), with sensitivity and specificity of 79.17 % (95%CI: 64.60%–89.04 %) and 88.68 % (95%CI: 76.28%–95.31 %).

Conclusions

CD69 and HLA-DR on host T and NK cells are promising markers in distinguishing different TB infection status. Our blood-based TB-Flow scoring system can distinguish ATB from LTBI with good diagnostic efficacy.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

在 QFT-TB 检测中同时检测宿主 T 细胞和 NK 细胞上的 CD69 和 HLA-DR 对识别活动性结核病的鉴别诊断价值

背景针对结核病（TB）的干扰素-γ释放测定（IGRA）在区分活动性肺结核（ATB）和潜伏性肺结核感染（LTBI）方面的能力仍然有限。目前，宿主 T 细胞和 NK 细胞上的活化标志物被认为是诊断 ATB 的有希望的标志物。方法这项前瞻性观察研究共招募了 213 名参与者，并将参与者分为 ATB 组、LTBI 组、其他肺部相关疾病组（ORD）和健康对照组（HC）。结果 ATB患者的活化标志物（CD69和HLA-DR）表达明显增加。NK 细胞上的 HLA-DR、T 细胞上的 CD69 和 QFT-TB 在 ATB 和 HC 的鉴别诊断中均具有良好的诊断价值（AUC>0.90）。此外，TB-Flow 还大大提高了 ATB 和 LTBI 的鉴别诊断效率（AUC=0.90，95%CI：0.84-0.96），敏感性和特异性分别为 79.17 %（95%CI：64.60%-89.04 %）和 88.68 %（95%CI：76.28%-95.31 %）。我们基于血液的 TB-Flow 评分系统可以区分 ATB 和 LTBI，并具有良好的诊断效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Tuberculosis 医学-呼吸系统

CiteScore

4.60

自引率

3.10%

发文量

审稿时长

49 days

期刊介绍： Tuberculosis is a speciality journal focusing on basic experimental research on tuberculosis, notably on bacteriological, immunological and pathogenesis aspects of the disease. The journal publishes original research and reviews on the host response and immunology of tuberculosis and the molecular biology, genetics and physiology of the organism, however discourages submissions with a meta-analytical focus (for example, articles based on searches of published articles in public electronic databases, especially where there is lack of evidence of the personal involvement of authors in the generation of such material). We do not publish Clinical Case-Studies. Areas on which submissions are welcomed include: -Clinical TrialsDiagnostics- Antimicrobial resistance- Immunology- Leprosy- Microbiology, including microbial physiology- Molecular epidemiology- Non-tuberculous Mycobacteria- Pathogenesis- Pathology- Vaccine development. This Journal does not accept case-reports. The resurgence of interest in tuberculosis has accelerated the pace of relevant research and Tuberculosis has grown with it, as the only journal dedicated to experimental biomedical research in tuberculosis.