Multiple resistance of Colletotrichum truncatum from soybean to QoI and MBC fungicides in Brazil

Abstract

Colletotrichum truncatum, the predominant fungal species associated with soybean anthracnose, is responsible for significant losses in this crop. Chemical control via fungicide application is the most effective strategy for the control of soybean foliar diseases. However, the increasing incidence of anthracnose in some regions of Brazil indicates that current chemical control is not effective against anthracnose. In this study, we evaluated the fungicide sensitivity of C. truncatum genetic lineages to the fungicides azoxystrobin, thiophanate-methyl, difenoconazole, and fludioxonil using isolates representing two important regions of soybean production in Brazil. We characterized the molecular resistance to the quinone-outside inhibitors (QoI), methyl benzimidazole carbamates (MBC), and demethylation inhibitor (DMI) fungicide groups based on amino acid sequences of the cytochrome b (cytb), β-tubulin gene (β-tub), and P450 sterol 14a-demethylases (CYP51) genes. Multiple resistance of C. truncatum isolates to QoI and MBC was observed associated with mutation points in the β-tub (E198A and F200Y) and cytb (G143A). Alternatively, low EC₅₀ values were found for fludioxonil and difenoconazole indicating high efficacy. Analysis of C. truncatum genomes revealed two potential DMI targets, CYP51A and CYP51B, and higher genetic variability in the CYP51A gene. A positive correlation was found between genetic differentiation of C. truncatum populations and fungicide sensitivity (Student's t-test <0.001). To our knowledge, this is the first report of multiple resistance to QoI and MBC fungicides in C. truncatum in Brazil.