A new endophytic Penicillium oxalicum with aphicidal activity and its infection mechanism.

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2024-07-03 DOI:10.1002/ps.8288

Yuxin Song, Xiaoli Liu, Kangbo Zhao, Ruyi Ma, Wenjun Wu, Yuanyuan Zhang, Longfei Duan, Xinnuo Li, Hong Xu, Min Cheng, Baofu Qin, Zhijun Qi

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Abstract

Background: Aphid infestation adversely affects the yield and quality of crops. Rapid reproduction and insecticidal resistance have made controlling aphids in the field challenging. Therefore, the present study investigated the insecticidal property of Penicillium oxalicum (QLhf-1) and its mechanism of action against aphids, Hyalopterus arundimis Fabricius.

Results: Bioassay revealed that the control efficacy of the spores against aphids (86.30% and 89.05% on the third day and fifth day after infection, respectively) were higher than other components, such as the mycelium. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that QLhf-1 invaded the aphid cuticle through spores and used the aphid tissues as a nutrient source for growth and reproduction, causing stiffness and atrophy and a final death. Three extracellular enzymes, lipase, protease, and chitinase had a synergistic effect with spores, and they acted together to complete the infection process by degrading the aphid body wall and accelerating the infection process.

Conclusion: The newly discovered endophytic penicillin strain P. oxalicum 'QLhf-1' can effectively kill aphids. The results provided strong evidence for the biological control of aphids, and lay a foundation for the development and utilization of QLhf-1. © 2024 Society of Chemical Industry.

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一种具有杀虫活性的新型草青霉内生菌及其感染机制。

背景：蚜虫为害对农作物的产量和质量造成了不利影响。蚜虫的快速繁殖和抗药性使田间蚜虫防治面临挑战。因此，本研究调查了草腐青霉（QLhf-1）的杀虫特性及其对蚜虫（Hyalopterus arundimis Fabricius）的作用机制：生物测定显示，孢子对蚜虫的防治效果（感染后第三天和第五天分别为 86.30% 和 89.05%）高于菌丝体等其他成分。扫描电子显微镜（SEM）和透射电子显微镜（TEM）显示，QLhf-1 通过孢子侵入蚜虫的角质层，并以蚜虫组织为营养源进行生长和繁殖，导致蚜虫组织僵硬、萎缩并最终死亡。脂肪酶、蛋白酶和几丁质酶三种胞外酶与孢子具有协同作用，它们共同降解蚜虫体壁，加速感染过程，从而完成感染过程：结论：新发现的内生青霉素菌株 P. oxalicum 'QLhf-1' 能有效杀死蚜虫。结果为蚜虫的生物防治提供了有力证据，并为 QLhf-1 的开发利用奠定了基础。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.