Nuclear factor kappa-light-chain-enhancer of activated B cells gene expression involvement in porcine liver transplant experimental model.

Acta cirurgica brasileira Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI:10.1590/acb392724

Lucas Souto Nacif, Flávio Galvão, Márcia Saldanha Kubrusly, Leonardo Yuri Kasputis Zanini, Paola Espinoza, Daniel Reis Waisberg, Rafael Soares Nunes Pinheiro, Amadeo Batista da Silva Neto, Vinicius Rocha-Santos, Venâncio Avancini Ferreira Alves, Luiz Carneiro-D'Albuquerque, Wellington Andraus

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Abstract

Purpose: Gene expressions of vascular Endothelial Growth Factor Alpha (VEGFa), Nuclear Factor Kappa-Light-Chain-Enhancer of Activated B cells (NFkB) and cytokines could be useful for identifying potential therapeutic targets to alleviate ischemia-reperfusion injury after liver transplantation. Cytokine gene expressions, VEGFa and NFkB were investigated in a preclinical swine model of liver transplantation.

Methods: A total of 12 pigs were used as donors and recipients in liver transplantation without venovenous bypass or aortic clamping. NFkB, IL-6, IL-10, VEGFa and Notch1 gene expression were assessed. These samples were collected in two specific times: group 1 (n= 6) - control, samples were collected before recipient's total hepatectomy and group 2 - liver transplantation group (n=6), where the samples were collected one hour after graft reperfusion.

Results: Liver transplantation was successfully performed in all recipients. Liver enzymes were elevated in the transplantation group. NFkB gene expression was significantly decreased in the transplantation group in comparison with the control group (0.62±0.19 versus 0.39±0.08; p= 0.016). No difference was observed between groups Interleucine 6 (IL-6), interleucine 10 (IL-10), VEGFa and Notch homolog 1 (Notch1).

Conclusions: In this survey a decreased NFkB gene expression in a porcine model of liver transplantation was observed.

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猪肝移植实验模型中活化 B 细胞核因子卡巴轻链增强子基因表达的参与。

目的：血管内皮生长因子α（VEGFa）、活化B细胞的核因子卡巴轻链增强因子（NFkB）和细胞因子的基因表达有助于确定潜在的治疗靶点，以减轻肝移植后的缺血再灌注损伤。我们在猪肝移植临床前模型中研究了细胞因子基因表达、血管内皮生长因子a和NFkB：方法：共使用 12 头猪作为肝移植的供体和受体，不进行静脉旁路或主动脉夹闭。评估了 NFkB、IL-6、IL-10、VEGFa 和 Notch1 基因的表达。这些样本在两个特定时间采集：第一组（n=6）--对照组，样本在受体全肝切除前采集；第二组--肝移植组（n=6），样本在移植物再灌注后一小时采集：结果：所有受者都成功进行了肝移植手术。结果：所有受者都成功进行了肝移植，移植组的肝酶升高。与对照组相比，移植组 NFkB 基因表达明显下降（0.62±0.19 对 0.39±0.08；P= 0.016）。白细胞介素6（IL-6）、白细胞介素10（IL-10）、血管内皮生长因子a和Notch同源物1（Notch1）各组之间未观察到差异：本次调查观察到猪肝移植模型中 NFkB 基因表达减少。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta cirurgica brasileira

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