Occurrence and genotyping of Enterocytozoon bieneusi in flying squirrels (Trogopterus xanthipes) from China.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-01-01 Epub Date: 2024-07-04 DOI:10.1051/parasite/2024037
Xuehan Liu, Chi Zhang, Tiantian Li, Xiaojing Xia, Yanzhao Xu, Jianhe Hu, Longxian Zhang, Lei Wang, Meng Qi
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Abstract

Enterocytozoon bieneusi is an obligate intracellular microsporidian parasite with a worldwide distribution. As a zoonotic pathogen, E. bieneusi can infect a wide range of wildlife hosts through the fecal-oral route. Although the feces of flying squirrels (Trogopterus xanthipes) are considered a traditional Chinese medicine (as "faeces trogopterori"), no literature is available on E. bieneusi infection in flying squirrels to date. In this study, a total of 340 fresh flying squirrel fecal specimens from two captive populations were collected in Pingdingshan city, China, to detect the prevalence of E. bieneusi and assess their zoonotic potential. By nested PCR amplification of the ITS gene, six specimens tested positive, with positive samples from each farm, with an overall low infection rate of 1.8%. The ITS sequences revealed three genotypes, including known genotype D and two novel genotypes, HNFS01 and HNFS02. Genotype HNFS01 was the most prevalent (4/6, 66.7%). Phylogenetic analysis showed that all genotypes clustered into zoonotic Group 1, with the novel genotypes clustering into different subgroups. To our knowledge, this is the first report of E. bieneusi infection in flying squirrels, suggesting that flying squirrels could act as a potential reservoir and zoonotic threat for E. bieneusi transmission to humans in China.

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中国鼯鼠(Trogopterus xanthipes)肠道酵母菌(Enterocytozoon bieneusi)的出现和基因分型。
生物肠孢子虫(Enterocytozoon bieneusi)是一种强制性胞内微孢子虫寄生虫,分布于世界各地。作为一种人畜共患病病原体,E. bieneusi 可通过粪-口途径感染多种野生动物宿主。虽然鼯鼠(Trogopterus xanthipes)的粪便被认为是一种传统中药(如 "鼯鼠粪"),但迄今为止还没有关于鼯鼠感染 E. bieneusi 的文献。本研究从中国平顶山市的两个人工饲养鼯鼠种群中采集了 340 份新鲜鼯鼠粪便标本,以检测 E. bieneusi 的感染率并评估其人畜共患病的可能性。通过巢式 PCR 扩增 ITS 基因,6 份标本检测结果呈阳性,其中每个养殖场均有阳性样本,总体感染率较低,仅为 1.8%。ITS 序列显示了三种基因型,包括已知的基因型 D 和两种新型基因型 HNFS01 和 HNFS02。基因型 HNFS01 的感染率最高(4/6,66.7%)。系统进化分析表明,所有基因型都被归入人畜共患病组 1,而新型基因型则被归入不同的亚组。据我们所知,这是首例鼯鼠感染E. bieneusi的报道,表明鼯鼠可能是E. bieneusi在中国传播给人类的潜在储库和人畜共患病威胁。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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