Production and Extraction of Red Bioactive Pigment from Streptomyces griseorubiginosus and Formulation of Bio-Lip Balm

Abstract

The current study is focused on the screening and isolation of a bioactive pigment producing actinomycete from paddy fields of Kerala, India. Among the five-pigment producing isolates obtained, a red colour pigmented actinomycetes was selected for further extraction and analysis. Optimization of production media enhanced the production of pigmented biomass. Glucose and beef extract was found to have a significant effect in the pigment production. The isolated actinomycetes was identified as Streptomyces griseorubiginosus by using 16 s r-DNA sequencing. Streptomyces griseorubiginosus cells were ruptured using ultrasonication and pigment was extracted using methanol as solvent. Different bio active compounds present in the extract were determined using Gas Chromatography Mass Spectrometry (GCMS) analysis and functional groups were identified using Fourier Transform Infrared (FT-1R) spectroscopy. Bioactive compounds present in the pigment showed significant antioxidant and antibacterial activities. The extracted red coloured pigment was used as a natural colorant to formulate a bio-lip balm. Preliminary analysis confirmed the stability of the synthesised bio-lip balm. Further, the extracted pigment can be used as a safer alternative for synthetic chemical pigments in various cosmetics, personal care products and pharmaceuticals.