Integrated Analysis of Transcriptome and Metabolome Reveal the Enhancement of Methyl Dihydrojasmonate on Physiological Indicators and Polyphyllins Biosynthesis in Paris polyphylla var. yunnanensis

IF 3.9 3区 生物学 Q1 PLANT SCIENCES Journal of Plant Growth Regulation Pub Date : 2024-07-02 DOI:10.1007/s00344-024-11413-9
Zihao Li, Yongbin Gu, Zixuan Hu, Furi Zhang, Yi Li, Mengqi Tian, Weijun Tian, Weirong Tian, Jiaru Li
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Abstract

Jasmonic acids, their effects on plant resistance and metabolites, have been extensively researched. However, methyl dihydrojasmonate (MDJ), a cost-effective and safe substitute for jasmonic acids, lacks sufficient research. This study presents a comprehensive analysis the impact of MDJ on Paris polyphylla var. yunnanensis. The present study aimed to enhance the accumulation of steroidal saponins (polyphyllin I, II, III, D, V, VI, VII, H) in Paris polyphylla var. yunnanensis which is a medicinal plant that contains medicinal steroidal saponins. To achieve this, different concentrations of MDJ were applied to Paris polyphylla var. yunnanensis. The study examined the impact of MDJ on physiology and polyphyllins. The optimal treatment concentration was identified, and the effects of MDJ on physiological metabolism and the expression pattern of related genes were explored. The results indicated that MDJ treatment significantly increased the levels of photosynthetic pigments, antioxidant enzymes, osmoregulators peaked at M2. Moreover, MDJ enhanced the accumulation of polyphyllins in both the leaves and rhizomes peaked at M2, particularly the sum of four polyphyllins (polyphyllin I, II, VI, VII) stated in the Chinese Pharmacopoeia, which increased by 45.65% following treatment. Based on transcriptome analysis, we revealed the polyphyllins-related genes by WGCNA. Furthermore, the majority of genes related to steroidal saponins were up-regulated following MDJ treatment. In the future, we recommend treating Paris polyphylla var. yunnanensis with suitable MDJ concentrations in production to efficiently enhance the yield of steroidal saponins.

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转录组和代谢组的综合分析揭示了二氢茉莉酸甲酯对云南巴黎绿变种生理指标和多花素合成的促进作用
茉莉酸及其对植物抗性和代谢物的影响已得到广泛研究。然而,作为茉莉酸的一种经济、安全的替代品,二氢茉莉酸甲酯(MDJ)还缺乏足够的研究。本研究全面分析了 MDJ 对云南白药的影响。本研究旨在提高含有药用甾体皂苷的药用植物云南白药(Paris polyphylla var.yunnanensis)中甾体皂苷(polyphyllin I、II、III、D、V、VI、VII、H)的积累。为此,对云南白药施用了不同浓度的 MDJ。该研究考察了 MDJ 对生理机能和多叶素的影响。确定了最佳处理浓度,并探讨了 MDJ 对生理代谢和相关基因表达模式的影响。结果表明,MDJ 处理能显著提高光合色素、抗氧化酶和渗透调节剂的水平,在 M2 浓度时达到峰值。此外,MDJ 还增强了多酚素在叶片和根茎中的积累,在 M2 时达到峰值,尤其是《中国药典》中记载的四种多酚素(多酚素 I、II、VI、VII)的总和在处理后增加了 45.65%。基于转录组分析,我们通过 WGCNA 发现了多酚素相关基因。此外,大多数与甾体皂苷相关的基因在 MDJ 治疗后上调。今后,我们建议在生产中使用适当浓度的 MDJ 处理云南白药,以有效提高甾体皂苷的产量。
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来源期刊
CiteScore
8.40
自引率
6.20%
发文量
312
审稿时长
1.8 months
期刊介绍: The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches. The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress. In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports. The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.
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