Urinary soluble PD-1 as a biomarker of Checkpoint Inhibitor induced acute tubulointerstitial nephritis

Abstract

Background Acute interstitial nephritis (AIN) related to Immune Checkpoint Inhibitors (ICI-AIN) has a not completely understood pathophysiology. Our objectives were to analyze possible biomarkers for the differentiation between acute tubular necrosis (ATN) and AIN, especially in cancer patients, and to study the participation of the immune checkpoint pathway in ICI-AIN. Methods We performed an observational study. We recruited patients with incident diagnosis of ICI-AIN (n=19). We measured soluble PD-1 (sPD-1), sPD-L1 and sPD-L2 in serum and urine at diagnosis and compared to patients with non ICI-related AIN (non-ICI AIN) (n=18) and ATN (n=21). The findings were validated in an independent cohort from another institution (n=30). Besides, we performed PD-L1 and PD-L2 immunostaining of kidney biopsies from patients with ICI-AIN and compared to patients with non-ICI AIN. Results Urinary sPD-1 (usPD-1) was higher in patients with AIN compared to ATN (p=0.03). Patients with AIN also showed higher serum sPD-1 (ssPD-1) than patients with ATN (p=0.021). In cancer patients, usPD-1 <129.3 pg/ml had a 71.43% sensitivity and 94.44% specificity to differentiate ATN from ICI-AIN, with a likelihood ratio of 12.86. In the external validation cohort, the same cutoff showed a sensitivity of 80%. In kidney biopsies, patients with ICI-AIN showed higher density of PD-L1 positive tubules than patients with non-ICI AIN (p=0.02). The proportion of patients having more than 2.64/mm2 PD-L2 positive tubules was higher among patients with ICI-AIN compared to non-ICI AIN (p=0.034). There was a positive correlation (p=0.009, r=0.72) between usPD-1 and the number of PD-L1 positive tubules. Conclusions UsPD-1 and ssPD-1 are higher in AIN than ATN. Moreover, there was a strong correlation between usPD-1 and renal tubular PD-L1 expression. Our findings suggest a role of usPD-1 as non-invasive biomarker to differentiate ICI-AIN from ATN, especially in cancer patients, which has been confirmed in an external validation cohort.