Design of a Liquid Cell for Structural Investigations of Supported Lipid Membrane by Synchrotron X-Rays Over a Wide Temperature Range, from Lower to Higher than Ambient
Avijit Das, Rajendra P. Giri, Mrinmay K. Mukhopadhyay
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引用次数: 0
Abstract
An oxygen-free copper-based cell has been designed to perform X-ray scattering experiments from the solid-supported lipid membranes immersed in water. This cell can be used to investigate the structural changes in the membrane depending on the composition of the lipids, the effects of temperatures, and the changes in arrangements of the lipids in the membrane on the addition of the external molecules. The design of the cell has been optimized to get a good signal-to-noise ratio (SNR) for the measurements of X-ray reflectivity and grazing incidence diffraction by controlling the X-ray path within the liquid in the cell and easy operation for variable liquid temperature. This very low-cost temperature-controlled setup, developed in our laboratory, can perform in situ X-ray measurements at various temperature ranges—starting from around 4°C up to 70°C which is very relevant to study the phase transition temperature of the lipids constituting the cell membranes. The detailed mechanical design of the cell and electrical control mechanism of the temperature of the liquid inside the cell has been discussed here. One such X-ray reflectivity measurement is shown here to express the capability of the cell for the study of the solid-supported bilayer sample composed of dipalmitoyl phosphocholine lipid at two different temperatures, below its phase transition temperature.
利用同步辐射 X 射线在从低于环境温度到高于环境温度的宽温度范围内对支撑脂膜进行结构研究的液态池设计
摘要 设计了一种无氧铜基电池，用于对浸入水中的固体支撑脂质膜进行 X 射线散射实验。该样品池可用于研究膜的结构变化取决于脂质的组成、温度的影响以及外部分子加入时膜中脂质排列的变化。对样品池的设计进行了优化，通过控制样品池液体中的 X 射线路径，在测量 X 射线反射率和掠入射衍射时获得了良好的信噪比（SNR），并且易于操作以改变液体温度。我们实验室开发的这种温控装置成本非常低，可以在从 4°C 左右到 70°C 的各种温度范围内进行原位 X 射线测量，这与研究构成细胞膜的脂质的相变温度非常相关。这里讨论了细胞的详细机械设计和细胞内液体温度的电气控制机制。其中一个 X 射线反射率测量结果表明，该样品池能够在低于相变温度的两个不同温度下研究由二棕榈酰基磷酸胆碱脂质组成的固态支撑双分子层样品。
期刊介绍：
Instruments and Experimental Techniques is an international peer reviewed journal that publishes reviews describing advanced methods for physical measurements and techniques and original articles that present techniques for physical measurements, principles of operation, design, methods of application, and analysis of the operation of physical instruments used in all fields of experimental physics and when conducting measurements using physical methods and instruments in astronomy, natural sciences, chemistry, biology, medicine, and ecology.
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