Assessment of lipid profile and antioxidant activity of pulp, shell, and kernel of Moroccan Balanites aegyptiaca fruit

Abstract

The Balanites aegyptiaca tree is widely used in many countries where it occurs. However, in Morocco its nutritional properties and chemical composition have not been extensively studied. This study aims to investigate its potential health benefits, focusing on bioactive compounds found in the fruit, including the pulp, shell and kernel. The fatty acid composition, phytosterol content, triacylglycerols and tocopherol levels of the lipid fraction extracted from different parts of Balanites fruit were analyzed using chromatographic techniques. Additionally, the antioxidant activity was assessed through the DPPH, ABTS, and FRAP assays. Results showed that the pulp, shell and kernel are rich in oleic acid, with percentages of 47.2%, 45.9%, and 41.9%, respectively. The linoleic acid content was consistent across all three parts. The shell had the highest phytosterols content, with β-sitosterol being the most abundant in all three parts. The analysis revealed some variability in the identification of tocopherols, with the kernel containing the highest content of tocopherols, mainly α-Tocopherol at 399 mg kg⁻¹. The fruit, in its entirety, exhibits antioxidant activity as evaluated by three different methods: DPPH, ABTS, and FRAP. Overall, the study demonstrates that the fruit of B. aegyptiaca growing in Morocco possesses properties that could be utilized in the food and medical industries.

Practical Application: The fruit parts of the Balanites tree (Balanites aegyptiaca) are rich in fatty acids, tocochromanols, and phytosterols, exhibiting significant antioxidant capacity. This study reveals the potential of the bioactive compounds of the fruit, which offers substantial practical applications as a natural source of antioxidants in food formulations and nutraceuticals. By leveraging the bioactive compounds identified in this study, researchers and industries can investigate the development of functional food and cosmeceuticals formulations to enhance human health and well-being.