LncRNA BBOX1-AS1 Contributes to Laryngeal Carcinoma Progression by Recruiting SRSF1 to Maintain EFNB2 mRNA Stability.

IF 2.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemical Genetics Pub Date : 2024-07-04 DOI:10.1007/s10528-024-10879-2

Xiaowen Zhu, Xuan Li, MeiJia Zhang, Jian Ni

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Abstract

Laryngeal cancer is a common malignancy of the larynx with a generally poor prognosis. This study systematically assessed the functional role of lncRNA BBOX1-AS1 in laryngeal carcinoma progression and associated molecular regulatory mechanisms. The proliferation, migration, and invasion of laryngeal carcinoma cells were detected by Cell Counting Kit-8, wound healing, clonal formation, and transwell assays. In addition, the interaction between BBOX1-AS1, Serine/Arginine Splicing Factor 1 (SRSF1), and Ephrin-B2 (EFNB2) mRNA was examined employing RNA immunoprecipitation and RNA pull-down experiments. Furthermore, western blotting, and RT-qPCR assays were adopted to detect the expression levels of BBOX1-AS1, SRSF1, and EFNB2. The impact of BBOX1-AS1 and SRSF1 on EFNB2 mRNA stability was examined using the RNA stability assay. BBOX1-AS1 was highly expressed in human laryngeal carcinoma tissues and cell lines. BBOX1-AS1 knockdown suppressed the growth, proliferation, migration, and invasion of laryngeal carcinoma cells. BBOX1-AS1 maintained the stability of EFNB2 mRNA in laryngeal carcinoma cells by recruiting SRSF1. EFNB2 knockdown inhibited the growth and metastatic function of laryngeal carcinoma cells in vitro. EFNB2 overexpression reversed the influence of BBOX1-AS1 knockdown on laryngeal cancer tumorigenesis. BBOX1-AS1 maintained EFNB2 mRNA stability by recruiting SRSF1, thereby aggravating laryngeal carcinoma malignant phenotypes. BBOX1-AS1 might be a new theoretical target for the treatment of laryngeal carcinoma.

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LncRNA BBOX1-AS1 通过招募 SRSF1 来维持 EFNB2 mRNA 的稳定性，从而促进喉癌的进展。

喉癌是一种常见的喉部恶性肿瘤，预后普遍较差。本研究系统评估了lncRNA BBOX1-AS1在喉癌进展中的功能作用及相关分子调控机制。通过细胞计数试剂盒-8、伤口愈合、克隆形成和透孔实验检测了喉癌细胞的增殖、迁移和侵袭。此外，还利用 RNA 免疫沉淀和 RNA pull-down 实验检测了 BBOX1-AS1、丝氨酸/精氨酸剪接因子 1 (SRSF1) 和 Ephrin-B2 (EFNB2) mRNA 之间的相互作用。此外，还采用了 Western 印迹法和 RT-qPCR 法检测 BBOX1-AS1、SRSF1 和 EFNB2 的表达水平。利用 RNA 稳定性检测法研究了 BBOX1-AS1 和 SRSF1 对 EFNB2 mRNA 稳定性的影响。BBOX1-AS1在人类喉癌组织和细胞系中高表达。BBOX1-AS1 基因敲除抑制了喉癌细胞的生长、增殖、迁移和侵袭。BBOX1-AS1 通过招募 SRSF1 维持喉癌细胞中 EFNB2 mRNA 的稳定性。EFNB2 基因敲除抑制了喉癌细胞在体外的生长和转移功能。EFNB2过表达可逆转BBOX1-AS1敲除对喉癌肿瘤发生的影响。BBOX1-AS1通过招募SRSF1维持EFNB2 mRNA的稳定性，从而加重喉癌的恶性表型。BBOX1-AS1可能是治疗喉癌的一个新的理论靶点。

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来源期刊

Biochemical Genetics 生物-生化与分子生物学

CiteScore

3.90

自引率

0.00%

发文量

133

审稿时长

4.8 months

期刊介绍： Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.