Silibinin improved the function of T cells in peripheral blood mononuclear cells (PBMCs) co-cultured with U-87 MG cell line.

IF 1.9 Q3 CHEMISTRY, MEDICINAL Avicenna Journal of Phytomedicine Pub Date : 2024-03-01 DOI:10.22038/AJP.2023.22935
Banafshe Abadi, Jahangir Abdesheikhi, Farnaz Sedghy, Merat Mahmoodi, Hossein Fallah
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Abstract

Objective: Silibinin has exhibited antitumor activities. However, there are few reports about the immunomodulatory properties of silibinin on T lymphocyte function in the tumor microenvironment. Here, we determined the effects of silibinin on T cells of peripheral blood mononuclear cells (PBMCs), cultivated alone or with a human cell line of glioblastoma (U-87 MG).

Materials and methods: The proliferation of T lymphocytes was assessed by MTT test in the presence of silibinin (15 and 45 µM). Also, total antioxidant capacity (TAC), the activity of superoxide dismutase-3 (SOD3), and the levels of two cytokines interferon gamma (IFN-γ) and tumor growth beta (TGF-β) were compared between treated and untreated PBMCs alone or co-cultured with U-87 cells.

Results: According to our results, silibinin raised the TAC levels and SOD3 activity in the PBMCs and in the co-culture condition. Moreover, silibinin-treated PBMCs showed higher IFN-γ levels and lower TGF-β levels. Interestingly, silibinin protected PBMCs against the U-87-induced suppression.

Conclusion: Altogether, these results proposed the immunomodulatory potential of silibinin on T cells of PBMCs, as well as its partially protective effects on PBMCs against the suppression induced by U-87 MG cells.

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Silibinin 可改善与 U-87 MG 细胞株共培养的外周血单核细胞(PBMC)中 T 细胞的功能。
目的Silibinin 具有抗肿瘤活性。然而,有关水飞蓟宾对肿瘤微环境中 T 淋巴细胞功能的免疫调节特性的报道却很少。在此,我们测定了西利宾对单独培养或与人胶质母细胞瘤细胞系(U-87 MG)一起培养的外周血单核细胞(PBMCs)T细胞的影响:在有西利宾(15 和 45 µM)存在的情况下,用 MTT 试验评估 T 淋巴细胞的增殖情况。此外,还比较了单独或与 U-87 细胞共培养的 PBMC 的总抗氧化能力(TAC)、超氧化物歧化酶-3(SOD3)的活性以及两种细胞因子γ干扰素(IFN-γ)和肿瘤生长β因子(TGF-β)的水平:结果:西利宾提高了PBMCs和共培养条件下的TAC水平和SOD3活性。此外,西利宾处理的 PBMCs 表现出更高的 IFN-γ 水平和更低的 TGF-β 水平。有趣的是,西利宾能保护 PBMC 免受 U-87 引起的抑制:总之,这些结果表明了西利宾对 PBMCs T 细胞的免疫调节潜力,以及对 PBMCs 免受 U-87 MG 细胞抑制的部分保护作用。
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来源期刊
Avicenna Journal of Phytomedicine
Avicenna Journal of Phytomedicine CHEMISTRY, MEDICINAL-
CiteScore
3.40
自引率
4.50%
发文量
17
审稿时长
6 weeks
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