Respiratory Viruses Co-detection on a Multiplex RT-PCR Panel: a Comparative Clinical Study.

IF 0.7 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY Clinical laboratory Pub Date : 2024-07-01 DOI:10.7754/Clin.Lab.2024.231232

Qute Choi, Hyun Jin Kim

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Abstract

Background: The FilmArray Respiratory Panel RP 2.1 plus (FilmArray RP) is a point-of-care syndromic panel for respiratory pathogens. Although highly valuable in the clinical settings, the co-detection of pathogens in FilmArray RP may confound result interpretation.

Methods: Nasopharyngeal swab specimens collected from patients with respiratory symptoms were analyzed by comparing co-detection results from FilmArray RP with those of Allplex Respiratory Panels (Allplex RP: Power-Chek for SARS-CoV-2).

Results: Out of 765 FilmArray RP tests, 143 (18.7%) showed co-detections (two: 122 (85.3%), three: 18 (12.6%), four: 2 (1.4%), and five viruses: 1 (0.7%). The most frequent co-detection was human rhinovirus/enterovirus (HRV/HEV) with respiratory syncytial virus (RSV) (22.3%, 32/143). The overall discordance rate between Film-Array RP and other tests was 32.9%. Notably, discordance in detecting adenovirus (AdV) was significant, with cases detected by FilmArray often not appearing in Allplex RP.

Conclusions: Discordances were varied by virus combination. It is advisable to perform additional confirmatory testing based on clinical relevance.

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在多重 RT-PCR 检测板上同时检测呼吸道病毒：一项临床比较研究。

背景：FilmArray Respiratory Panel RP 2.1 plus（FilmArray RP）是一种针对呼吸道病原体的床旁综合分析仪。尽管 FilmArray RP 具有很高的临床价值，但病原体的共同检测可能会混淆结果的解释：方法：通过比较 FilmArray RP 与 Allplex Respiratory Panels（Allplex RP：Power-Chek for SARS-CoV-2）的共同检测结果，对从有呼吸道症状的患者身上采集的鼻咽拭子标本进行分析：结果：在 765 次 FilmArray RP 检测中，有 143 次（18.7%）出现了共同检测结果（两种病毒：122 次（85.3%），三种病毒：18 次（12.6%），四种病毒：2 次（1.4%），五种病毒：1 次（0.7%））：1 (0.7%).最常见的共同检测是人鼻病毒/肠道病毒（HRV/HEV）与呼吸道合胞病毒（RSV）（22.3%，32/143）。Film-Array RP 与其他检测方法的总体不一致率为 32.9%。值得注意的是，腺病毒（AdV）检测的不一致性非常明显，FilmArray 检测出的病例往往不会出现在 Allplex RP 中：结论：病毒组合不同，不一致性也不同。建议根据临床相关性进行额外的确证检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical laboratory 医学-医学实验技术

CiteScore

1.50

自引率

0.00%

发文量

494

审稿时长

3 months

期刊介绍： Clinical Laboratory is an international fully peer-reviewed journal covering all aspects of laboratory medicine and transfusion medicine. In addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies. The journal publishes original articles, review articles, posters, short reports, case studies and letters to the editor dealing with 1) the scientific background, implementation and diagnostic significance of laboratory methods employed in hospitals, blood banks and physicians'' offices and with 2) scientific, administrative and clinical aspects of transfusion medicine and 3) in addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies.