Zinc-glutathione mitigates alcohol-induced intestinal and hepatic injury by modulating intestinal zinc-transporters in mice

IF 4.8 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Nutritional Biochemistry Pub Date : 2024-07-02 DOI:10.1016/j.jnutbio.2024.109697
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Abstract

Long-term alcohol overconsumption impairs intestinal and hepatic structure and function, along with dysregulation of zinc homeostasis. We previously found that zinc-glutathione (Zn-GSH) complex effectively suppressed alcohol-induced liver injury in mice. This study was undertaken to test the hypothesis that Zn-GSH suppresses alcohol-induced liver injury by modulating intestinal zinc transporters. Mice were subjected to long-term ethanol feeding, as per the NIAAA model, with groups receiving either an ethanol diet alone or an ethanol diet supplemented with Zn-GSH. Treatment groups were carefully monitored for alcohol consumption and subjected to a final binge drinking exposure. The results showed that Zn-GSH increased the survival rate and decreased the recovery time from binge drinking-induced drunkenness. Histopathological analyses demonstrated a reduction in liver steatosis and the preservation of intestinal integrity by Zn-GSH. It was observed that Zn-GSH prevented the reduction of Zn and GSH levels while increasing alcohol dehydrogenase and aldehyde dehydrogenase in both liver and intestine. Importantly, the expression and protein abundance of zinc transporters ZnT-1, ZIP-1, ZIP-4, ZIP-6, and ZIP-14, all of which are critically involved in intestinal zinc transport and homeostasis, were significantly increased or preserved by Zn-GSH in response to alcohol exposure. This study thus highlights the critical role of Zn-GSH in maintaining intestinal zinc homeostasis by modulating zinc transporters, thereby preventing alcohol-induced intestinal and hepatic injury.

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锌-谷胱甘肽通过调节小鼠肠道锌转运体减轻酒精引起的肠道和肝损伤。
长期过量饮酒会损害肠道和肝脏的结构和功能,并导致锌平衡失调。我们以前曾发现锌-谷胱甘肽(Zn-GSH)复合物能有效抑制酒精引起的小鼠肝损伤。本研究旨在验证 Zn-GSH 通过调节肠道锌转运体抑制酒精诱导的肝损伤的假设。按照 NIAAA 模型,对小鼠进行长期乙醇喂养,各组分别接受单纯乙醇饮食或补充 Zn-GSH 的乙醇饮食。对治疗组的酒精消耗量进行仔细监测,并对其进行最后的暴饮处理。结果表明,Zn-GSH 提高了暴饮引起的醉酒的存活率并缩短了恢复时间。组织病理学分析表明,Zn-GSH 降低了肝脏脂肪变性,保护了肠道完整性。研究还发现,Zn-GSH 在防止 Zn 和 GSH 水平降低的同时,还能增加肝脏和肠道中的酒精脱氢酶和醛脱氢酶。重要的是,锌转运体 ZnT-1、ZIP-1、ZIP-4、ZIP-6 和 ZIP-14 的表达量和蛋白丰度都因 Zn-GSH 而显著增加或保持不变。因此,这项研究强调了 Zn-GSH 在通过调节锌转运体维持肠道锌平衡方面的关键作用,从而防止酒精引起的肠道和肝损伤。
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来源期刊
Journal of Nutritional Biochemistry
Journal of Nutritional Biochemistry 医学-生化与分子生物学
CiteScore
9.50
自引率
3.60%
发文量
237
审稿时长
68 days
期刊介绍: Devoted to advancements in nutritional sciences, The Journal of Nutritional Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology. Rigorous reviews by an international editorial board of distinguished scientists ensure publication of the most current and key research being conducted in nutrition at the cellular, animal and human level. In addition to its monthly features of critical reviews and research articles, The Journal of Nutritional Biochemistry also periodically publishes emerging issues, experimental methods, and other types of articles.
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