Designing and developing a programmable in vitro gastrointestinal model to assess the impact of pasteurization on the bioaccessibility of lycopene nanoparticles

IF 5.3 2区农林科学 Q1 ENGINEERING, CHEMICAL Journal of Food Engineering Pub Date : 2024-07-01 DOI:10.1016/j.jfoodeng.2024.112211

M. Anwar Ul Alam , L. Kassama

{"title":"Designing and developing a programmable in vitro gastrointestinal model to assess the impact of pasteurization on the bioaccessibility of lycopene nanoparticles","authors":"M. Anwar Ul Alam , L. Kassama","doi":"10.1016/j.jfoodeng.2024.112211","DOIUrl":null,"url":null,"abstract":"<div>To evaluate potential health impact of engineered nanoparticles, evaluation of digestion effect is necessary. Digestion requires micro-management and sequential execution of complex enzymatic mechanisms with controlled human digestion environment. The objective of this study was to develop an automated in-vitro digestive system to assess bio-accessibility of encapsulated lycopene nanoparticles in model fruit juice. Lycopene was encapsulated in polylactic acid (PLA-LNP) and polylactic co-glycolic acid (PLGA-LNP) and evaluated the bio-accessibility in a programmable in-vitro human digestive system operated with high sensitivity (25–30μs) and volume accuracy (97.37–100%). Pasteurized Model juice containing PLA-LNP, revealing higher bio-accessibility (100%) compared to PLGA-LNP (73%) counterparts. Absorption profile of both types of nanoparticles followed Korsmeyer-Peppas diffusion model at R2 value of 0.98. All types of nanoparticles with different treatments followed non-Fickian types of diffusion except PLGA-PLA with microwave pasteurization. This automated in-vitro digestive system holds significant potential for advancing drug discovery and delivery studies in the future.</div>","PeriodicalId":359,"journal":{"name":"Journal of Food Engineering","volume":null,"pages":null},"PeriodicalIF":5.3000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Engineering","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0260877424002772","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}

引用次数: 0

Abstract

To evaluate potential health impact of engineered nanoparticles, evaluation of digestion effect is necessary. Digestion requires micro-management and sequential execution of complex enzymatic mechanisms with controlled human digestion environment. The objective of this study was to develop an automated in-vitro digestive system to assess bio-accessibility of encapsulated lycopene nanoparticles in model fruit juice. Lycopene was encapsulated in polylactic acid (PLA-LNP) and polylactic co-glycolic acid (PLGA-LNP) and evaluated the bio-accessibility in a programmable in-vitro human digestive system operated with high sensitivity (25–30μs) and volume accuracy (97.37–100%). Pasteurized Model juice containing PLA-LNP, revealing higher bio-accessibility (100%) compared to PLGA-LNP (73%) counterparts. Absorption profile of both types of nanoparticles followed Korsmeyer-Peppas diffusion model at R² value of 0.98. All types of nanoparticles with different treatments followed non-Fickian types of diffusion except PLGA-PLA with microwave pasteurization. This automated in-vitro digestive system holds significant potential for advancing drug discovery and delivery studies in the future.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

设计和开发可编程体外胃肠道模型，以评估巴氏杀菌法对番茄红素纳米粒子生物可及性的影响

要评估工程纳米粒子对健康的潜在影响，就必须评估消化效果。消化需要微观管理和在可控的人体消化环境下按顺序执行复杂的酶解机制。本研究的目的是开发一种自动体外消化系统，以评估模型果汁中封装番茄红素纳米粒子的生物可及性。番茄红素被封装在聚乳酸（PLA-LNP）和聚乳酸共聚乙酸（PLGA-LNP）中，并在一个可编程的体外人体消化系统中评估其生物可及性，该系统具有高灵敏度（25-30μs）和体积准确性（97.37-100%）。含有聚乳酸-LNP 的巴氏杀菌模型果汁的生物可接受性（100%）高于聚乳酸-LNP（73%）。两种纳米粒子的吸收曲线都遵循 Korsmeyer-Peppas 扩散模型，R2 值为 0.98。除采用微波巴氏灭菌法的 PLGA-PLA 外，所有经不同处理的纳米粒子都遵循非菲克扩散类型。这种自动体外消化系统在未来推进药物发现和递送研究方面具有巨大潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of Food Engineering 工程技术-工程：化工

CiteScore

11.80

自引率

5.50%

发文量

275

审稿时长

24 days

期刊介绍： The journal publishes original research and review papers on any subject at the interface between food and engineering, particularly those of relevance to industry, including: Engineering properties of foods, food physics and physical chemistry; processing, measurement, control, packaging, storage and distribution; engineering aspects of the design and production of novel foods and of food service and catering; design and operation of food processes, plant and equipment; economics of food engineering, including the economics of alternative processes. Accounts of food engineering achievements are of particular value.