CircFAM114A2 Suppresses Cell Proliferation, Migration, and Invasion of Colorectal Cancer Through Sponging miR-647 to Upregulate DAB2IP Expression.

IF 2.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemical Genetics Pub Date : 2024-07-06 DOI:10.1007/s10528-024-10870-x

Guanghao Huang, Dahua Sun, Xiaoli Hu, Qiushuang Wang

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引用次数: 0

Abstract

Background: Increasing evidence had proved that some circular RNA (circRNA) exerted critical roles in tumors progression by functioning as "microRNAs (miRNAs) sponges" to regulate their targeted genes.

Methods: circFAM114A2 and miR-647 expression was measured in CRC tissues and cells by quantitative real-time polymerase chain reaction (qRT-PCR), and the prognostic value of circFAM114A2 evaluated by Kaplan-Meier survival curve. Subsequently, wounding healing and transwell assays were performed to assess cell proliferation, migration, and invasion. RNA pull-down and dual-luciferase reporter assays were used to confirm the interactions between circFAM114A2, miR-647, and DAB2IP.

Results: CircFAM114A2 was notably downregulated in CRC tissues and cells, and low circFAM114A2 expression indicated the poor prognosis of CRC patients. Next, overexpression of circFAM114A2 suppressed CRC cells proliferation, migration, and invasion in vitro and impede CRC tumor growth in vivo. Mechanically, circFAM114A2 competitively bound to miR-647 and upregulated its target gene DAB2IP expression in CRC cells.

Conclusion: Our results indicated that circFAM114A2/miR-647/DAP2IP axis played an important role in CRC progression, suggesting that circFAM114A2 might be a novel therapeutic target in patients with CRC.

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CircFAM114A2 通过海绵状 miR-647 上调 DAB2IP 表达，抑制结直肠癌细胞增殖、迁移和侵袭

背景：方法：通过实时聚合酶链式反应（qRT-PCR）定量检测CRC组织和细胞中circFAM114A2和miR-647的表达，并通过Kaplan-Meier生存曲线评估circFAM114A2的预后价值。随后进行了伤口愈合和透孔试验，以评估细胞的增殖、迁移和侵袭。采用 RNA pull-down 和双荧光素酶报告实验证实了 circFAM114A2、miR-647 和 DAB2IP 之间的相互作用：结果：circFAM114A2在CRC组织和细胞中明显下调，circFAM114A2的低表达表明CRC患者预后不良。其次，过表达 circFAM114A2 可抑制体外 CRC 细胞的增殖、迁移和侵袭，并阻碍体内 CRC 肿瘤的生长。在机制上，circFAM114A2与miR-647竞争性结合，并上调其靶基因DAB2IP在CRC细胞中的表达：我们的研究结果表明，circFAM114A2/miR-647/DAP2IP轴在CRC进展过程中发挥了重要作用，这表明circFAM114A2可能是CRC患者的一个新的治疗靶点。

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来源期刊

Biochemical Genetics 生物-生化与分子生物学

CiteScore

3.90

自引率

0.00%

发文量

133

审稿时长

4.8 months

期刊介绍： Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.