Recombinant Human IL-32θ Induces Polarization Into M1-like Macrophage in Human Monocytic Cells.

IF 4.3 4区 医学 Q2 IMMUNOLOGY Immune Network Pub Date : 2024-06-24 eCollection Date: 2024-06-01 DOI:10.4110/in.2024.24.e27
Hyo-Min Park, Jae-Young Park, Na-Yeon Kim, Hyemoon Kim, Hong-Gyum Kim, Dong-Ju Son, Jin Tae Hong, Do-Young Yoon
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Abstract

The tumor microenvironment (TME) is formed by several immune cells. Notably, tumor-associated macrophages (TAMs) are existed in the TME that induce angiogenesis, metastasis, and proliferation of cancer cells. Recently, a point-mutated variant of IL-32θ was discovered in breast cancer tissues, which suppressed migration and proliferation through intracellular pathways. Although the relationship between cancer and IL-32 has been previously studied, the effects of IL-32θ on TAMs remain elusive. Recombinant human IL-32θ (rhIL-32θ) was generated using an Escherichia coli expression system. To induce M0 macrophage polarization, THP-1 cells were stimulated with PMA. After PMA treatment, the cells were cultured with IL-4 and IL-13, or rhIL-32θ. The mRNA level of M1 macrophage markers (IL-1β, TNFα, inducible nitric oxide synthase) were increased by rhIL-32θ in M0 macrophages. On the other hand, the M2 macrophage markers (CCL17, CCL22, TGFβ, CD206) were decreased by rhIL-32θ in M2 macrophages. rhIL-32θ induced nuclear translocation of the NF-κB via regulation of the MAPK (p38) pathway. In conclusion, point-mutated rhIL-32θ induced the polarization to M1-like macrophages through the MAPK (p38) and NF-κB (p65/p50) pathways.

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重组人 IL-32θ 可诱导人单核细胞极化为 M1 样巨噬细胞。
肿瘤微环境(TME)由多种免疫细胞组成。值得注意的是,肿瘤相关巨噬细胞(TAMs)存在于肿瘤微环境中,诱导血管生成、转移和癌细胞增殖。最近,在乳腺癌组织中发现了 IL-32θ 的点突变变体,它通过细胞内途径抑制迁移和增殖。虽然癌症与 IL-32 之间的关系此前已有研究,但 IL-32θ 对 TAMs 的影响仍然难以捉摸。重组人 IL-32θ(rhIL-32θ)是利用大肠杆菌表达系统产生的。为诱导 M0 巨噬细胞极化,用 PMA 刺激 THP-1 细胞。PMA 处理后,用 IL-4 和 IL-13 或 rhIL-32θ 培养细胞。rhIL-32θ 能提高 M0 巨噬细胞中 M1 巨噬细胞标志物(IL-1β、TNFα、诱导型一氧化氮合酶)的 mRNA 水平。rhIL-32θ 通过调节 MAPK(p38)通路诱导 NF-κB 核转位。总之,点突变的 rhIL-32θ 通过 MAPK(p38)和 NF-κB (p65/p50)途径诱导巨噬细胞极化为 M1 样巨噬细胞。
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来源期刊
Immune Network
Immune Network Immunology and Microbiology-Immunology
CiteScore
2.90
自引率
3.30%
发文量
36
期刊介绍: Immune Network publishes novel findings in basic and clinical immunology and aims to provide a medium through which researchers in various fields of immunology can share and connect. The journal focuses on advances and insights into the regulation of the immune system and the immunological mechanisms of various diseases. Research that provides integrated insights into translational immunology is given preference for publication. All submissions are evaluated based on originality, quality, clarity, and brevity
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