Involvement of D1 dopamine receptor in the nucleus of the solitary tract of rats in stress-induced hypertension and exercise.

IF 3.3 2区 医学 Q1 PERIPHERAL VASCULAR DISEASE Journal of Hypertension Pub Date : 2024-10-01 Epub Date: 2024-07-04 DOI:10.1097/HJH.0000000000003809
Ko Yamanaka, Makoto Suzuki, Linh Thuy Pham, Keisuke Tomita, Thu Van Nguyen, Miwa Takagishi, Kei Tsukioka, Sabine Gouraud, Hidefumi Waki
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Abstract

Objective: Chronic stress can cause hypertension, whereas daily exercise promotes healthy well being through destressing. Although the nucleus of the solitary tract (NTS) is involved in the development of hypertension, the molecular and physiological mechanisms of stress and exercise remain unclear. In this study, we tested whether gene expression in the NTS is altered by stress and daily exercise and whether this is involved in cardiovascular regulation.

Methods: We have performed RT 2 Profiler PCR arrays targeting a panel of neurotransmitter receptor genes in the NTS of Wistar rats subjected to chronic restraint stress (1 h a day over 3 weeks) with or without voluntary wheel exercise. We also performed immunohistochemistry to determine whether the identified molecules were expressed at the protein level. Additionally, microinjection studies in anesthetized rats were performed to examine whether validated molecules exhibit physiological roles in cardiovascular regulation of the NTS.

Results: We observed that blood pressure was significantly increased by stress and the increase was suppressed by exercise. Using PCR analysis, we determined that the expression levels of four genes in the NTS, including the dopamine receptor D1 gene ( Drd1 ), were significantly affected by stress and suppressed by exercise. We also examined dopamine D1 receptor (D1R) expression in NTS neurons and found significantly greater expression in the stressed than nonstressed animals. Furthermore, the microinjection of a D1R agonist into the NTS in anesthetized rats induced hypotensive effects.

Conclusion: These results suggest that NTS D1R plays a role in the counteracting processes of stress-induced hypertension.

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大鼠孤束核D1多巴胺受体参与应激诱发的高血压和运动
目的慢性压力可导致高血压,而日常锻炼则可通过消除压力促进健康。虽然孤束核(NTS)与高血压的发生有关,但压力和运动的分子和生理机制仍不清楚。在这项研究中,我们测试了 NTS 的基因表达是否会因压力和日常运动而改变,以及这是否参与了心血管调节:方法:我们对接受慢性束缚应激(每天 1 小时,持续 3 周)并进行或不进行自主车轮运动的 Wistar 大鼠的 NTS 进行了 RT2 Profiler PCR 阵列检测,检测了一系列神经递质受体基因。我们还进行了免疫组化,以确定所识别的分子是否在蛋白质水平上表达。此外,我们还在麻醉大鼠体内进行了显微注射研究,以检验验证的分子是否在 NTS 的心血管调节中发挥生理作用:结果:我们观察到,压力会使血压明显升高,而运动会抑制血压的升高。通过 PCR 分析,我们确定 NTS 中包括多巴胺受体 D1 基因 (Drd1) 在内的四个基因的表达水平受到应激的显著影响,并受到运动的抑制。我们还检测了多巴胺 D1 受体(D1R)在 NTS 神经元中的表达,发现应激动物的多巴胺 D1 受体表达明显高于非应激动物。此外,向麻醉大鼠的 NTS 显微注射 D1R 激动剂可诱导降血压效应:这些结果表明,NTS D1R在应激诱导的高血压的对抗过程中发挥作用。
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来源期刊
Journal of Hypertension
Journal of Hypertension 医学-外周血管病
CiteScore
7.90
自引率
6.10%
发文量
1389
审稿时长
3 months
期刊介绍: The Journal of Hypertension publishes papers reporting original clinical and experimental research which are of a high standard and which contribute to the advancement of knowledge in the field of hypertension. The Journal publishes full papers, reviews or editorials (normally by invitation), and correspondence.
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