Dispel some mist on circulating biopterins: measurement, physiological interval and pathophysiological implication.

IF 3.5 3区医学 Q2 ENDOCRINOLOGY & METABOLISM Metabolomics Pub Date : 2024-07-09 DOI:10.1007/s11306-024-02137-8

Hai-Bo Wang, Xuan Xiao, Wen Dai, Yan Cui, Wan-Man Li, Rui Peng, Liu Hu, Shao-Ting Wang

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Abstract

Background and aims: Biopterins, including tetrahydrobiopterin (BH4), dihydrobiopterin (BH2), and biopterin (B), were crucial enzyme cofactors in vivo. Despite their recognized clinical significance, there remain notable research gaps and controversies surrounding experimental outcomes. This study aims to clarify the biopterins-related issues, including analytical art, physiological intervals, and pathophysiological implications.

Materials and methods: A novel LC-MS/MS method was developed to comprehensively profile biopterins in plasma, utilizing chemical derivatization and cold-induced phase separation. Subsequently, apparently healthy individuals were enrolled to investigate the physiological ranges. And the relationships between biopterins and biochemical indicators were analyzed to explore the pathophysiological implications.

Results: The developed method was validated as reliable for detecting biopterins across the entire physiological range. Timely anti-oxidation was found to be essential for accurate assessment of biopterins. The observed overall mean ± SDs levels were 3.51 ± 0.94, 1.54 ± 0.48, 2.45 ± 0.84 and 5.05 ± 1.14 ng/mL for BH4, BH2, BH4/BH2 and total biopterins. The status of biopterins showed interesting correlations with age, gender, hyperuricemia and overweight.

Conclusion: In conjunction with proper anti-oxidation, the newly developed method enables accurate determination of biopterins status in plasma. The observed physiological intervals and pathophysiological implications provide fundamental yet inspiring support for further clinical researches.

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揭开循环生物蝶呤的迷雾：测量、生理间隔和病理生理学意义。

背景和目的：生物蝶呤，包括四氢生物蝶呤 (BH4)、二氢生物蝶呤 (BH2) 和生物蝶呤 (B)，是体内重要的酶辅助因子。尽管生物蝶呤的临床意义已得到公认，但围绕其实验结果的研究仍存在明显差距和争议。本研究旨在澄清生物蝶呤相关问题，包括分析技术、生理间隔和病理生理影响：利用化学衍生化和冷诱导相分离技术，开发了一种新的 LC-MS/MS 方法来全面分析血浆中的生物蝶呤。随后，对明显健康的人进行了研究，以调查其生理范围。结果表明，所开发的方法对血浆中的生物蝶呤进行了可靠的检测：结果：经验证，所开发的方法可在整个生理范围内可靠地检测生物蝶呤。及时抗氧化对准确评估生物蝶呤至关重要。观察到的 BH4、BH2、BH4/BH2 和总生物蝶呤的总体平均值（± SD）水平分别为 3.51 ± 0.94、1.54 ± 0.48、2.45 ± 0.84 和 5.05 ± 1.14 纳克/毫升。生物蝶呤的状况与年龄、性别、高尿酸血症和超重有有趣的相关性：结论：新开发的方法与适当的抗氧化相结合，能准确测定血浆中生物蝶呤的状态。所观察到的生理间隔和病理生理学意义为进一步的临床研究提供了基础性的、鼓舞人心的支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolomics 医学-内分泌学与代谢

CiteScore

6.60

自引率

2.80%

发文量

审稿时长

2 months

期刊介绍： Metabolomics publishes current research regarding the development of technology platforms for metabolomics. This includes, but is not limited to: metabolomic applications within man, including pre-clinical and clinical pharmacometabolomics for precision medicine metabolic profiling and fingerprinting metabolite target analysis metabolomic applications within animals, plants and microbes transcriptomics and proteomics in systems biology Metabolomics is an indispensable platform for researchers using new post-genomics approaches, to discover networks and interactions between metabolites, pharmaceuticals, SNPs, proteins and more. Its articles go beyond the genome and metabolome, by including original clinical study material together with big data from new emerging technologies.