AtSNU13 modulates pre-mRNA splicing of RBOHD and ALD1 to regulate plant immunity.

IF 4.4 1区生物学 Q1 BIOLOGY BMC Biology Pub Date : 2024-07-10 DOI:10.1186/s12915-024-01951-9

Yanke Jiang, Yingzhe Yue, Chongchong Lu, Muhammad Zunair Latif, Haifeng Liu, Zhaoxu Wang, Ziyi Yin, Yang Li, Xinhua Ding

{"title":"AtSNU13 modulates pre-mRNA splicing of RBOHD and ALD1 to regulate plant immunity.","authors":"Yanke Jiang, Yingzhe Yue, Chongchong Lu, Muhammad Zunair Latif, Haifeng Liu, Zhaoxu Wang, Ziyi Yin, Yang Li, Xinhua Ding","doi":"10.1186/s12915-024-01951-9","DOIUrl":null,"url":null,"abstract":"<p><p>Pre-mRNA splicing is a significant step for post-transcriptional modifications and functions in a wide range of physiological processes in plants. Human NHP2L binds to U4 snRNA during spliceosome assembly; it is involved in RNA splicing and mediates the development of human tumors. However, no ortholog has yet been identified in plants. Therefore, we report At4g12600 encoding the ortholog NHP2L protein, and AtSNU13 associates with the component of the spliceosome complex; the atsnu13 mutant showed compromised resistance in disease resistance, indicating that AtSNU13 is a positive regulator of plant immunity. Compared to wild-type plants, the atsnu13 mutation resulted in altered splicing patterns for defense-related genes and decreased expression of defense-related genes, such as RBOHD and ALD1. Further investigation shows that AtSNU13 promotes the interaction between U4/U6.U5 tri-snRNP-specific 27 K and the motif in target mRNAs to regulate the RNA splicing. Our study highlights the role of AtSNU13 in regulating plant immunity by affecting the pre-mRNA splicing of defense-related genes.</p>","PeriodicalId":9339,"journal":{"name":"BMC Biology","volume":null,"pages":null},"PeriodicalIF":4.4000,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11234627/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s12915-024-01951-9","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Pre-mRNA splicing is a significant step for post-transcriptional modifications and functions in a wide range of physiological processes in plants. Human NHP2L binds to U4 snRNA during spliceosome assembly; it is involved in RNA splicing and mediates the development of human tumors. However, no ortholog has yet been identified in plants. Therefore, we report At4g12600 encoding the ortholog NHP2L protein, and AtSNU13 associates with the component of the spliceosome complex; the atsnu13 mutant showed compromised resistance in disease resistance, indicating that AtSNU13 is a positive regulator of plant immunity. Compared to wild-type plants, the atsnu13 mutation resulted in altered splicing patterns for defense-related genes and decreased expression of defense-related genes, such as RBOHD and ALD1. Further investigation shows that AtSNU13 promotes the interaction between U4/U6.U5 tri-snRNP-specific 27 K and the motif in target mRNAs to regulate the RNA splicing. Our study highlights the role of AtSNU13 in regulating plant immunity by affecting the pre-mRNA splicing of defense-related genes.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

AtSNU13 可调节 RBOHD 和 ALD1 的前核糖核酸剪接，从而调控植物免疫。

前 mRNA 剪接是转录后修饰的一个重要步骤，在植物的多种生理过程中发挥着作用。人类 NHP2L 在剪接体组装过程中与 U4 snRNA 结合；它参与 RNA 剪接并介导人类肿瘤的发生。然而，在植物中尚未发现同源物。因此，我们报道了编码直向同源物 NHP2L 蛋白的 At4g12600，以及 AtSNU13 与剪接体复合物组分的关联；atsnu13 突变体在抗病性方面表现出了妥协性，表明 AtSNU13 是植物免疫的正向调节因子。与野生型植物相比，atsnu13突变导致防御相关基因的剪接模式发生改变，RBOHD和ALD1等防御相关基因的表达量减少。进一步的研究表明，AtSNU13能促进U4/U6.U5三nRNP特异性27 K与目标mRNA中的motif相互作用，从而调控RNA的剪接。我们的研究强调了 AtSNU13 通过影响防御相关基因的前 mRNA 剪接在调节植物免疫中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

BMC Biology 生物-生物学

CiteScore

7.80

自引率

1.90%

发文量

260

审稿时长

3 months

期刊介绍： BMC Biology is a broad scope journal covering all areas of biology. Our content includes research articles, new methods and tools. BMC Biology also publishes reviews, Q&A, and commentaries.