Exploring the venom of Ectatomma brunneum Smith (Hymenoptera: Formicidae)

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-07-06 DOI:10.1007/s00049-024-00407-4

Rafaella Caroline Bernardi, Angélica Mendonça, Ellen Liciane Barbosa Firmino, Luis Fernando Benitez Macorini, Edvaldo Barros, Pollyanna Pereira Santos, Luiz Carlos Santos-Junior, José Eduardo Serrão, William Fernando Antonialli-Junior, Claudia Andréa Lima Cardoso

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Abstract

Ant venom has several functions, including predation, communication, defense against predators, and action against pathogens. There is a scarcity of data about ant venom components that could provide support for understanding the mechanisms of action. The objective here was to identify the amino acids and proteins in the venom of the predatory ant Ectatomma brunneum and to evaluate its antimicrobial activity. The amino acids were analyzed by liquid chromatography, with diode array detection, and were identified using amino acid standards. The two-dimensional (2D) gel electrophoresis fractionation approach was used to identify the proteins, together with MALDI-TOF/TOF mass spectrometry and protein databases. The antimicrobial activity of the venom was evaluated using the minimum inhibitory and minimum microbiocidal concentrations. The venom of E. brunneum contained free amino acids, with a high amount of alanine. The 2D gel analysis showed 104 spots, of which 21 were identified and classified according to biological function, as follows: venom proteins, nontoxic reservoir protection, cellular maintenance proteins, and proteins with unknown function. The venom showed antimicrobial activity, inhibiting the growth of all the bacteria and fungi tested. The results provide new insights into ant venom components and antimicrobial activity.

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探究布氏外蝠（膜翅目：蝠科）的毒液

蚂蚁毒液具有多种功能，包括捕食、交流、抵御天敌和对抗病原体。有关蚂蚁毒液成分的数据非常稀少，而这些数据可以为了解蚂蚁毒液的作用机制提供支持。本文的目的是鉴定捕食蚁 Ectatomma brunneum 毒液中的氨基酸和蛋白质，并评估其抗菌活性。氨基酸采用液相色谱法和二极管阵列检测法进行分析，并使用氨基酸标准进行鉴定。采用二维（2D）凝胶电泳分馏法、MALDI-TOF/TOF 质谱法和蛋白质数据库对蛋白质进行鉴定。使用最小抑菌浓度和最小杀菌浓度评估了毒液的抗菌活性。布氏杆菌毒液含有游离氨基酸，其中丙氨酸含量较高。二维凝胶分析显示出 104 个斑点，其中 21 个已被确定，并根据生物功能分类如下：毒液蛋白、无毒储库保护蛋白、细胞维持蛋白和功能未知蛋白。毒液具有抗菌活性，可抑制所有受测细菌和真菌的生长。这些结果为了解蚂蚁毒液成分和抗菌活性提供了新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.