Analysis of viral diversity in dogs with acute gastroenteritis from Brazilian Amazon

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES Infection Genetics and Evolution Pub Date : 2024-07-08 DOI:10.1016/j.meegid.2024.105637

Danielle Rodrigues de Deus , Jones Anderson Monteiro Siqueira , Marcelino Antonio Costa Maués , Márcia Janete de Fátima Mesquita de Figueiredo , Edivaldo Costa Sousa Júnior , Renato da Silva Bandeira , Kenny da Costa Pinheiro , Dielle Monteiro Teixeira , Luciana Damascena da Silva , Sylvia de Fátima dos Santos Guerra , Luana da Silva Soares , Yvone Benchimol Gabbay

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Abstract

Viral gastroenteritis is commonly reported in dogs and involves a great diversity of enteric viruses. In this research, viral diversity was investigated in dogs with diarrhea in Northern Brazil using shotgun metagenomics. Furthermore, the presence of norovirus (NoV) was investigated in 282 stool/rectal swabs of young/adult dogs with or without diarrhea from two public kennels, based on one-step reverse transcription polymerase chain reaction (RT-PCR) for genogroup VI and VII (GVI and GVII) and real-time RT-PCR for GI, GII, and GIV. Thirty-one viral families were identified, including bacteriophages. Phylogenetic analyses showed twelve complete or nearly complete genomes belonging to the species of Protoparvovirus carnivoran1, Mamastrovirus 5, Aichivirus A2, Alphacoronavirus 1, and Chipapillomavirus 1. This is the first description of the intestinal virome of dogs in Northern Brazil and the first detection of canine norovirus GVII in the country. These results are important for helping to understand the viral groups that circulate in the canine population.

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巴西亚马逊地区急性肠胃炎患犬病毒多样性分析。

据报道，病毒性肠胃炎在狗中很常见，涉及多种多样的肠道病毒。在这项研究中，我们利用猎枪元基因组学研究了巴西北部腹泻犬的病毒多样性。此外，研究人员还通过一步式反转录聚合酶链反应（RT-PCR）检测基因组 VI 和 VII（GVI 和 GVII），以及实时 RT-PCR 检测 GI、GII 和 GIV，对来自两家公共犬舍的 282 只患腹泻或未患腹泻的幼犬/成年犬的粪便/直肠拭子中是否存在诺如病毒（NoV）进行了调查。共鉴定出 31 个病毒家族，其中包括噬菌体。系统进化分析表明，有 12 个完整或接近完整的基因组，分别属于 Protoparvovirus carnivoran1、Mamastrovirus 5、Aichivirus A2、Alphacoronavirus 1 和 Chipapillomavirus 1。这是首次对巴西北部犬肠道病毒组的描述，也是首次在巴西检测到犬诺如病毒 GVII。这些结果对于了解犬类中流行的病毒群非常重要。

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来源期刊

Infection Genetics and Evolution 医学-传染病学

CiteScore

8.40

自引率

0.00%

发文量

215

审稿时长

82 days

期刊介绍： (aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID) Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance. However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors. Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases. Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .