Validation of the HemoCue Hb 801 portable haemoglobin analyser for fish blood.

IF 1.7 3区 农林科学 Q2 FISHERIES Journal of fish biology Pub Date : 2025-01-01 Epub Date: 2024-07-11 DOI:10.1111/jfb.15871
Joana Henze, Sofia Kamperin, Eric Metsmaa, Lizeth Alcocer Arboleda, M Iranzu Calocany Aramendia, Erika Sundell, Ida Hedén, Niklas Warwas, Jonathan A C Roques
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Abstract

Haemoglobin concentration ([Hb]) assessment in fish blood has become a routine parameter to measure the health and welfare status of the animals. The original method (haemoglobincyanide method, best known as the Drabkin method) for measuring Hb in human and animals is not well suited for work outside of a laboratory setting. It is relatively time consuming, contains hazardous cyanide elements, and requires specific laboratory material. As an alternative to the Drabkin method, portable analysers have been developed for human blood, but they need to be first validated for fish blood before being used in experiments. In this study, the performance of the new HemoCue Hb 801 portable haemoglobin analyser was compared to the validated Drabkin method to determine [Hb] in three fish species. Hb readings between the two methods were not different for any of the species tested (rainbow trout, Onchorynchus mykiss, Atlantic wolffish, Anarhichas lupus, and Nile tilapia, Oreochromis niloticus). Therefore, this new portable device can be readily used to measure Hb in fish blood. Unlike the previous model from HemoCue, the Hb 201+, this device does not need an incubation time or a correction factor, representing a major gain of time and precision.

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HemoCue Hb 801 便携式鱼血血红蛋白分析仪的验证。
鱼类血液中的血红蛋白浓度([Hb])评估已成为衡量动物健康和福利状况的常规参数。测量人类和动物血红蛋白的原始方法(血红蛋白氰化物法,最著名的是 Drabkin 法)不太适合实验室以外的工作。这种方法相对耗时,含有危险的氰化物元素,并且需要特定的实验室材料。作为 Drabkin 方法的替代方法,已开发出用于人体血液的便携式分析仪,但在用于实验之前,需要首先对鱼类血液进行验证。本研究将新型 HemoCue Hb 801 便携式血红蛋白分析仪的性能与经过验证的 Drabkin 方法进行了比较,以测定三种鱼类的[Hb]。两种方法的血红蛋白读数对任何受测物种(虹鳟、大西洋狼鱼和尼罗罗非鱼)都没有差异。因此,这种新型便携式仪器可随时用于测量鱼类血液中的 Hb。与 HemoCue 以前的型号 Hb 201+ 不同,该设备不需要孵育时间或校正因子,大大节省了时间并提高了精度。
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来源期刊
Journal of fish biology
Journal of fish biology 生物-海洋与淡水生物学
CiteScore
4.00
自引率
10.00%
发文量
292
审稿时长
3 months
期刊介绍: The Journal of Fish Biology is a leading international journal for scientists engaged in all aspects of fishes and fisheries research, both fresh water and marine. The journal publishes high-quality papers relevant to the central theme of fish biology and aims to bring together under one cover an overall picture of the research in progress and to provide international communication among researchers in many disciplines with a common interest in the biology of fish.
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