Aberrant telomeric structures and serum markers of telomere dysfunction in healthy aging: a preliminary study.

IF 4.4 4区 医学 Q1 GERIATRICS & GERONTOLOGY Biogerontology Pub Date : 2024-11-01 Epub Date: 2024-07-13 DOI:10.1007/s10522-024-10120-y
Virginia Boccardi, Luigi Cari, Patrizia Bastiani, Michela Scamosci, Roberta Cecchetti, Giuseppe Nocentini, Patrizia Mecocci
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Abstract

Telomeres undergo a progressive shortening process as individuals age, and it has been proposed that severely shortened and dysfunctional telomeres play a role in the aging process and the onset of age-related diseases in human beings. An emerging body of evidence indicates that the shortening of telomeres in cultured human cells is also influenced by other replication defects occurring within telomeric repeats. These abnormalities can be detected on metaphase chromosomes. Recent studies have also identified a set of serological markers for telomere dysfunction and DNA damage (elongation factor 1α [EF-1α], stathmin, and N-acetyl-glucosaminidase). With this study, the correlation between telomere abnormalities (by FISH) and these biomarkers as measured in blood serum (by ELISA) from a cohort of 22 healthy subjects at different ages (range 26-101 years) was analyzed. A strong positive correlation between aging and the presence of aberrant telomere structures, sister telomere loss (STL), and sister telomere chromatid fusions (STCF) was detected. When serum markers of telomere dysfunction were correlated with telomere abnormalities, we found that stathmin correlated with total aberrant telomeres structures (r = 0.431, p = 0.0453) and STCF (r = 0.533, p = 0.0107). These findings suggest that serum stathmin can be considered an easy-to-get marker of telomere dysfunction and may serve as valuable indicators of aging.

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健康老龄化过程中端粒结构异常和端粒功能障碍的血清标志物:一项初步研究。
随着年龄的增长,端粒会逐渐缩短,有人认为端粒的严重缩短和功能障碍在人类衰老过程和与年龄相关疾病的发生中起着作用。越来越多的证据表明,培养的人类细胞中端粒的缩短也受到端粒重复序列中其他复制缺陷的影响。这些异常可在分裂期染色体上检测到。最近的研究还发现了一组端粒功能障碍和DNA损伤的血清学标记(伸长因子1α [EF-1α]、stathmin和N-乙酰葡糖苷酶)。通过这项研究,我们分析了端粒异常(通过 FISH 检测)与血清中这些生物标志物(通过 ELISA 检测)之间的相关性,这些生物标志物来自 22 名不同年龄段(26-101 岁)的健康受试者。结果发现,衰老与端粒结构异常、姐妹端粒缺失(STL)和姐妹端粒染色单体融合(STCF)之间存在很强的正相关性。当端粒功能障碍的血清标记物与端粒异常相关时,我们发现 stathmin 与端粒总异常结构(r = 0.431,p = 0.0453)和 STCF(r = 0.533,p = 0.0107)相关。这些研究结果表明,血清中的stathmin可被视为端粒功能障碍的一种易于获取的标记物,并可作为有价值的衰老指标。
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来源期刊
Biogerontology
Biogerontology 医学-老年医学
CiteScore
8.00
自引率
4.40%
发文量
54
审稿时长
>12 weeks
期刊介绍: The journal Biogerontology offers a platform for research which aims primarily at achieving healthy old age accompanied by improved longevity. The focus is on efforts to understand, prevent, cure or minimize age-related impairments. Biogerontology provides a peer-reviewed forum for publishing original research data, new ideas and discussions on modulating the aging process by physical, chemical and biological means, including transgenic and knockout organisms; cell culture systems to develop new approaches and health care products for maintaining or recovering the lost biochemical functions; immunology, autoimmunity and infection in aging; vertebrates, invertebrates, micro-organisms and plants for experimental studies on genetic determinants of aging and longevity; biodemography and theoretical models linking aging and survival kinetics.
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