The motile behavior of virus-transformed 3T3 cells.

Scanning electron microscopy Pub Date : 1986-01-01
G Albrecht-Buehler
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Abstract

Using metallic gold in various assays for the motility of cultured tissue cells, the paper compares the movements of surface projections and the locomotion of polyoma (Py3T3) and SV40 (SV3T3) virus-transformed 3T3 cells with the behavior of the parental 3T3 cells. The movement of surface projections was assayed by the ability of filopodia, lamellipodia and blebs of freshly plated cells to remove colloidal gold particles from a particle-coated glass substrate. The ability of filopodia to probe the environment for points of anchorage was tested by observing cells plated on glass whose filopodia touched the surface of a neighboring area of evaporated gold. The locomotion of cells was assayed by particle-free tracks (phagokinetic tracks) which were left by migrating cells on a glass substrate which was previously coated with colloidal gold particles. The paper suggests that the ability of the transformed cells to sense environmental factors, and their behavioral controls are altered.

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病毒转化3T3细胞的运动行为。
本文用金属金对培养组织细胞的运动进行了各种测定,比较了多瘤(Py3T3)和SV40 (SV3T3)病毒转化的3T3细胞与亲本3T3细胞的表面突起运动和运动。通过新镀细胞的丝状足、板状足和气泡去除颗粒涂层玻璃基板上的胶体金颗粒的能力,分析了表面突起的运动。通过观察镀在玻璃上的细胞,丝状足接触邻近蒸发金区域的表面,来测试丝状足探测环境锚定点的能力。细胞的运动是通过无颗粒轨迹(吞噬动力学轨迹)来检测的,这些轨迹是由迁移细胞在先前涂有胶体金颗粒的玻璃基板上留下的。本文认为,转化后的细胞感知环境因素的能力及其行为控制发生了改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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