Identification of hub lncRNAs correlated with tetralogy of fallot based on weighted gene co-expression network analysis

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemistry and Biophysics Reports Pub Date : 2024-07-13 DOI:10.1016/j.bbrep.2024.101786

Ye Ding , Chun Zhu

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Abstract

The most prevalent cyanotic congenital heart disease, Tetralogy of Fallot (TOF), has an unknown etiology. Long-stranded non-coding RNAs (lncRNAs) have been linked to cardiac development and congenital heart disease, as evidenced by an increasing number of studies; nevertheless, additional research is necessary to fully understand the function that TOF-related lncRNAs play in the condition. This study constructed lncRNA-mRNA co-expression networks, performed functional enrichment analysis, and screened hub lncRNAs using Weighted Gene Co-expression Network Analysis (WGCNA) using the Gene Expression Omnibus dataset GSE36761. Ten hub lncRNAs, including IRF1-AS1, AC012360.6, HLA-F-AS1, RP1-253P7.4, NPTN-IT1, RP11–166P13.4, RP5-1183I21.2, SNHG14, CH17-98J9.1, and RP11–894P9.1, were identified by WGCNA analysis as potentially significant contributors to the development of TOF. Based on functional enrichment analysis, lncRNA mainly contributes to TOF by altering gene splicing patterns. New insights on the mechanism underlying TOF occurrence are provided by identifying hub lncRNAs associated with the disease and analyzing their regulatory networks.

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基于加权基因共表达网络分析鉴定与法洛氏四联症相关的中枢lncRNAs

最常见的紫绀型先天性心脏病--法洛氏四联症（TOF）病因不明。越来越多的研究证明，长链非编码 RNA（lncRNA）与心脏发育和先天性心脏病有关；然而，要充分了解与 TOF 有关的 lncRNA 在该病中的功能，还需要更多的研究。本研究利用基因表达总库数据集GSE36761构建了lncRNA-mRNA共表达网络，进行了功能富集分析，并使用加权基因共表达网络分析（WGCNA）筛选了枢纽lncRNA。通过WGCNA分析，确定了10个中枢lncRNA，包括IRF1-AS1、AC012360.6、HLA-F-AS1、RP1-253P7.4、NPTN-IT1、RP11-166P13.4、RP5-1183I21.2、SNHG14、CH17-98J9.1和RP11-894P9.1，这些lncRNA可能对TOF的发展有重要影响。根据功能富集分析，lncRNA主要通过改变基因剪接模式导致TOF。通过识别与TOF相关的中枢lncRNA并分析其调控网络，为TOF的发生机制提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.