Integrated analysis of differentially m6A modified and expressed lncRNAs for biomarker identification in coronary artery disease

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2024-07-14 DOI:10.1002/cbin.12224

Rongli Jiang, Qiaowei Jia, Chengcheng Li, Xiongkang Gan, Yaqing Zhou, Yang Pan, Yahong Fu, Xiumei Chen, Lanyu Liang, Enzhi Jia

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Abstract

N6-methyladenosine (m6A) is the most prevalent internal RNA modification in mammals. However, limited research has been conducted on the role of m6A in coronary artery disease (CAD). We conducted methylated RNA immunoprecipitation sequencing and RNA sequencing to obtain a genome-wide profile of m6A-modified long noncoding RNAs (lncRNAs) in human coronary artery smooth muscle cells either exposed to oxidized low-density lipoprotein treatment or not, and the characteristics of the expression profiles were explored using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. The predictive effects of seven selected lncRNAs on CAD were evaluated in peripheral blood mononuclear cells (PBMCs). The differentially m6A-modified and expressed lncRNAs related genes were predominantly enriched in small GTPase-mediated signal transduction, ErbB signaling, and Rap1 signaling. Additionally, the expression levels of uc003pes.1, ENST00000422847, and NR_110155 were significantly associated with CAD, with uc003pes.1 identified as an independent risk factor and NR_110155 as an independent protective factor for CAD. NR_110155 and uc003pes.1 in PBMCs have the potential to serve as biomarkers for predicting CAD.

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综合分析不同 m6A 修饰和表达的 lncRNA，用于冠状动脉疾病的生物标记物鉴定。

N6-甲基腺苷（m6A）是哺乳动物体内最常见的内部 RNA 修饰。然而，有关 m6A 在冠状动脉疾病（CAD）中的作用的研究还很有限。我们通过甲基化 RNA 免疫沉淀测序和 RNA 测序，获得了暴露于氧化低密度脂蛋白处理或未暴露于氧化低密度脂蛋白处理的人冠状动脉平滑肌细胞中 m6A 修饰的长非编码 RNA（lncRNA）的全基因组图谱，并利用基因本体和京都基因和基因组百科全书分析探讨了表达图谱的特征。在外周血单核细胞（PBMC）中评估了所选的七个lncRNA对CAD的预测作用。经 m6A 修饰和表达的 lncRNAs 相关基因主要富集在小 GTPase 介导的信号转导、ErbB 信号转导和 Rap1 信号转导中。此外，uc003pes.1、ENST00000422847 和 NR_110155 的表达水平与 CAD 显著相关，其中 uc003pes.1 被确定为 CAD 的独立危险因素，而 NR_110155 则被确定为 CAD 的独立保护因素。PBMCs中的NR_110155和uc003pes.1有可能成为预测CAD的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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