Tropospheric ozone effect on olfactory perception and olfactory bulb dopaminergic interneuron excitability

Abstract

Ozone (O₃) forms in the Earth’s atmosphere, both naturally and by reactions of man-made air pollutants. Deleterious effects of O₃ have been found in the respiratory system. Here, we examine whether O₃ alters olfactory behavior and cellular properties in the olfactory system. For this purpose, mice were exposed to O₃ at a concentration found in highly polluted city air [0.8 ppm], and the behavior elicited by social and non-social odors in habituation/dishabituation tests was assessed. In addition, the electrical responses of dopaminergic olfactory bulb (OB) neurons were also evaluated. O₃ differentially compromises olfactory perception to odors: it reduces responses to social and non-social odors in Swiss Webster mice, while this effect was observed in C57BL/6 J mice only for some non-social odors. Additionally, O₃ reduced the rate of spontaneous spike firing in periglomerular dopaminergic cells (PG-DA) of the OB. Because this effect could reflect changes in excitability and/or synaptic inputs, the ability of O₃ to alter PG-DA spontaneous activity was also tested together with cell membrane resistance, membrane potential, rheobase and chronaxie. Taken together, our data suggest the ability of O₃ to affect olfactory perception.