A miniaturized QuEChERS and UPLC-MS/MS method for the determination of mycotoxins in cashew nuts.

IF 2.3 3区农林科学 Q2 CHEMISTRY, APPLIED Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment Pub Date : 2024-09-01 Epub Date: 2024-07-15 DOI:10.1080/19440049.2024.2376156

Felipe Stanislau Candido, Andre Victor Sartori, Armi Wanderley da Nobrega

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Abstract

This study aimed to develop and validate a multi-mycotoxin analysis method applied to cashew nuts by employing a miniaturized QuEChERS method followed by determination by ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Satisfactory recoveries for the concentrations 1, 10 and 30 ng g^-1, ranging from 66% (fumonisin B₁) to 110% (ochratoxin A) and relative standard deviations lower than 9% (fumonisin B₂) were obtained for the target compounds. Limits of quantification ranged from 0.004 ng g^-1 (sterigmatocystin) to 0.59 ng g^-1 (alternariol). The applicability of the analytical method was verified by analyzing 30 cashew nut samples from the city of Rio de Janeiro, RJ, southeastern Brazil. Aflatoxins M1, G2, G1, B2, B1, ochratoxin A and sterigmatocystin were detected, respectively, in 27%, 10%, 17%, 30%, 30%, 30% and 50% of the analyzed samples, at maximum concentrations of 0.56, 0.67, 1.43, 2.02, 4.93, 4.81, and 0.35 ng g^-1. The maximum limit established by Brazilian legislation for aflatoxins was not exceeded by any of the analyzed samples.

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测定腰果中霉菌毒素的微型 QuEChERS 和 UPLC-MS/MS 方法。

这项研究旨在开发和验证一种适用于腰果的多霉菌毒素分析方法，该方法采用微型 QuEChERS 法，然后用超高效液相色谱-串联质谱法（UHPLC-MS/MS）进行测定。目标化合物在 1、10 和 30 纳克/克浓度下的回收率为 66%（伏马菌素 B1）至 110%（赭曲霉毒素 A），相对标准偏差低于 9%（伏马菌素 B2）。定量限为 0.004 纳克/克-1（固形物胱氨酸）至 0.59 纳克/克-1（交替硫醇）。通过分析巴西东南部里约热内卢市的 30 份腰果样品，验证了该分析方法的适用性。在分析的样本中，分别有 27%、10%、17%、30%、30%、30% 和 50%的样本检测到黄曲霉毒素 M1、G2、G1、B2、B1、赭曲霉毒素 A 和固醇麦角菌素，最高浓度分别为 0.56、0.67、1.43、2.02、4.93、4.81 和 0.35 纳克/克。没有任何分析样本超过巴西法律规定的黄曲霉毒素最高限量。

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来源期刊

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment CHEMISTRY, APPLIED-FOOD SCIENCE & TECHNOLOGY

CiteScore

7.40

自引率

6.90%

发文量

136

审稿时长

3 months

期刊介绍： Food Additives & Contaminants: Part A publishes original research papers and critical reviews covering analytical methodology, occurrence, persistence, safety evaluation, detoxification and regulatory control of natural and man-made additives and contaminants in the food and animal feed chain. Papers are published in the areas of food additives including flavourings, pesticide and veterinary drug residues, environmental contaminants, plant toxins, mycotoxins, marine biotoxins, trace elements, migration from food packaging, food process contaminants, adulteration, authenticity and allergenicity of foods. Papers are published on animal feed where residues and contaminants can give rise to food safety concerns. Contributions cover chemistry, biochemistry and bioavailability of these substances, factors affecting levels during production, processing, packaging and storage; the development of novel foods and processes; exposure and risk assessment.