Insertion with long target duplication in polymyxin B-induced resistant mutant of Salmonella

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES Journal of global antimicrobial resistance Pub Date : 2024-07-14 DOI:10.1016/j.jgar.2024.07.002
Tongtong Zhang , Huifen Jiang , Ying Zhao , Tingting Yao , Rui Li
{"title":"Insertion with long target duplication in polymyxin B-induced resistant mutant of Salmonella","authors":"Tongtong Zhang ,&nbsp;Huifen Jiang ,&nbsp;Ying Zhao ,&nbsp;Tingting Yao ,&nbsp;Rui Li","doi":"10.1016/j.jgar.2024.07.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>A <em>Salmonella enterica</em> subsp. <em>diarizonae</em> (hereafter <em>S. diarizonae</em>) clinical strain S499 demonstrated unique genomic features. The strain S499 was treated with polymyxin B in vitro to investigate the mechanism of resistance.</p></div><div><h3>Methods</h3><p>S499 was treated with polymyxin B by increasing concentration gradually to obtain a resistant mutant S499V. Whole genomes of the two strains were sequenced using Illumina HiSeq X<strong>-</strong>10 and PacBio RS II platforms. Reverse transcription–quantitative polymerase chain reaction (RT-qPCR) was performed to compare the gene expression.</p></div><div><h3>Results</h3><p>The chromosome of strain S499 contained a 40-kb DNA region that was replicated after treatment with polymyxin B and generated a triple tandem DNA repeat region in the chromosome of mutant strain S499V. This repeat region in S499V was flanked by IS<em>1</em> and contained <em>pmrD, pmrG</em>, and <em>arnBCADTEF</em> operon<em>.</em> In comparison to the homologous 40-kb DNA region of strain S499, a few genes in the repeat DNA region of strain S499V contained truncating mutations that generate two open reading frames (ORFs). The expression of <em>pmrD, pmrG</em>, and <em>arnT</em> was significantly upregulated in S499V.</p></div><div><h3>Conclusion</h3><p>The duplication and overexpression of <em>pmrD, pmrG</em>, and <em>arnT</em> operon may be responsible for the polymyxin B resistance of mutant strain S499V.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"38 ","pages":"Pages 231-235"},"PeriodicalIF":3.7000,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001334/pdfft?md5=b614bd4747185be867b00655dbb4f751&pid=1-s2.0-S2213716524001334-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524001334","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

Abstract

Objectives

A Salmonella enterica subsp. diarizonae (hereafter S. diarizonae) clinical strain S499 demonstrated unique genomic features. The strain S499 was treated with polymyxin B in vitro to investigate the mechanism of resistance.

Methods

S499 was treated with polymyxin B by increasing concentration gradually to obtain a resistant mutant S499V. Whole genomes of the two strains were sequenced using Illumina HiSeq X-10 and PacBio RS II platforms. Reverse transcription–quantitative polymerase chain reaction (RT-qPCR) was performed to compare the gene expression.

Results

The chromosome of strain S499 contained a 40-kb DNA region that was replicated after treatment with polymyxin B and generated a triple tandem DNA repeat region in the chromosome of mutant strain S499V. This repeat region in S499V was flanked by IS1 and contained pmrD, pmrG, and arnBCADTEF operon. In comparison to the homologous 40-kb DNA region of strain S499, a few genes in the repeat DNA region of strain S499V contained truncating mutations that generate two open reading frames (ORFs). The expression of pmrD, pmrG, and arnT was significantly upregulated in S499V.

Conclusion

The duplication and overexpression of pmrD, pmrG, and arnT operon may be responsible for the polymyxin B resistance of mutant strain S499V.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
多粘菌素 B 诱导的沙门氏菌耐药突变体中的长目标重复插入。
目的:肠炎沙门氏菌 diarizonae 亚种(以下简称 diarizonae)临床菌株 S499 具有独特的基因组特征。在体外用多粘菌素 B 处理 S499 菌株,以研究其耐药性机制:方法:用多粘菌素 B 处理 S499,逐渐增加浓度,以获得抗性突变体 S499V。使用 Illumina HiSeq X-10 和 PacBio RS II 平台对两株菌株的全基因组进行测序。对基因表达进行了反转录定量 PCR(RT-qPCR)比较:结果:菌株 S499 的染色体包含一个 40 kb 的 DNA 区域,经多粘菌素 B 处理后复制,在突变菌株 S499V 的染色体中产生了一个三重串联 DNA 重复区域。S499V 的这一重复区两侧是 IS1,包含 pmrD、pmrG 和 arnBCADTEF 操作子。与菌株 S499 的同源 40-kb DNA 区域相比,菌株 S499V 重复 DNA 区域中的一些基因含有截短突变,产生了两个开放阅读框(ORF)。在 S499V 中,pmrD、pmrG 和 arnT 的表达显著上调:结论:pmrD、pmrG 和 arnT 操作子的重复和过度表达可能是突变株 S499V 对多粘菌素 B 产生抗性的原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
文献相关原料
公司名称产品信息其他信息采购帮参考价格
上海源叶 polymyxin B
¥60.00~¥32240.90
上海源叶 ciprofloxacin
¥16.00~¥24336.00
上海源叶 amoxicillin
¥23.00~¥23690.00
上海源叶 ampicillin
¥22.00~¥13501.00
上海源叶 ceftriaxone
¥150.00~¥7879.90
上海源叶 furantoin
¥33.90~¥6535.00
上海源叶 sulfisoxazole
¥30.00~¥6420.00
上海源叶 gentamicin
¥148.00~¥5938.00
上海源叶 chloramphenicol
¥19.00~¥4834.90
上海源叶 tetracyclines
来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
期刊最新文献
Surveillance and Characteristics of Vancomycin-Resistant Enterococcus Isolates in a Chinese Tertiary Hospital in Shenzhen, 2018 to 2024. Genomic analysis of IMP-8-producing Enterobacter hormaechei with a novel plasmid pK432-IMP. Monte Carlo simulation for dosage optimization of the best available therapy for bloodstream infections secondary to carbapenemase-producing Klebsiella pneumoniae in critically ill patients. Refining the gut colonization Zophobas morio larvae model using an oral administration of multidrug-resistant Escherichia coli. From Forgotten Cure to Modern Medicine: The Resurgence of Bacteriophage Therapy.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1