Expression of opsin and visual cycle-related enzymes in fetal rat skin keratinocytes and cellular response to blue light

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemistry and Biophysics Reports Pub Date : 2024-07-16 DOI:10.1016/j.bbrep.2024.101789
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Abstract

The mechanism by which the skin, a non-visual tissue, responds to light remains unknown. To date, opsin expression has been demonstrated in keratinocytes, melanocytes, and fibroblasts, all of which are skin-derived cells. In this study, we examined whether the visual cycle, by which opsin activity is maintained, is present in skin keratinocytes. We also identified the wavelengths of light to which opsin in keratinocytes responds and explored their effects on skin keratinocytes. The fetal rat skin keratinocytes used in this study expressed OPN2, 3, and 5 in addition to enzymes involved in the visual cycle, and all-trans-retinal, which is produced by exposure to light, was reconverted to 11-cis-retinal, resulting in opsin activation. Using the production of all-trans-retinal after light exposure as an indicator, we discovered that keratinocytes responded to light at 450 nm. Furthermore, actin alpha cardiac muscle 1 expression in keratinocytes was enhanced and cell migration was suppressed by exposure to light at these wavelengths. These results indicate that keratinocytes express various opsins and have a visual cycle that keeps opsin active. Moreover, keratinocytes were shown to respond to the blue/UV region of the light spectrum, suggesting that opsin plays a role in the light response of the skin.

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大鼠胎儿皮肤角质细胞中视紫红质和视觉周期相关酶的表达以及细胞对蓝光的反应
皮肤是一种非视觉组织,其对光的反应机制仍然未知。迄今为止,在角质形成细胞、黑色素细胞和成纤维细胞中都已证实有视蛋白的表达,而所有这些细胞都是源自皮肤的细胞。在这项研究中,我们研究了皮肤角质形成细胞中是否存在视觉周期,该周期是维持光学视蛋白活性的基础。我们还确定了角质形成细胞中的视蛋白对哪些波长的光有反应,并探讨了这些光对皮肤角质形成细胞的影响。本研究中使用的胎鼠皮肤角质形成细胞除了表达参与视觉循环的酶外,还表达 OPN2、3 和 5,并且在光照射下产生的全反式视网膜被重新转化为 11-顺式视网膜,从而激活了视蛋白。以光照射后产生的全反式视网膜为指标,我们发现角质细胞对 450 纳米波长的光有反应。此外,在这些波长的光照下,角质细胞中肌动蛋白α心肌1的表达增强,细胞迁移受到抑制。这些结果表明,角质形成细胞表达各种视蛋白,并有一个保持视蛋白活性的视觉周期。此外,研究还显示角质细胞对光谱中的蓝色/紫外线区域有反应,这表明蛋白酶在皮肤的光反应中发挥作用。
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来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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