Assessment of crAssphage as a biological variable for SARS-CoV-2 data normalization in wastewater surveillance.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of Applied Microbiology Pub Date : 2024-07-02 DOI:10.1093/jambio/lxae177

André Vinicius Costa Ribeiro, Camille Ferreira Mannarino, Shênia Patrícia Corrêa Novo, Tatiana Prado, André Lermontov, Bruna Barbosa de Paula, Tulio Machado Fumian, Marize Pereira Miagostovich

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引用次数: 0

Abstract

Aims: This study aimed to assess the use of cross-assembled phage (crAssphage) as an endogenous control employing a multivariate normalization analysis and its application as a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) data normalizer.

Methods and results: A total of 188 twelve-hour composite raw sewage samples were obtained from eight wastewater treatment plants (WWTP) during a 1-year monitoring period. Employing the N1 and N2 target regions, SARS-CoV-2 RNA was detected in 94% (177) and 90% (170) of the samples, respectively, with a global median of 5 log10 genomic copies per liter (GC l-1). CrAssphage was detected in 100% of the samples, ranging from 8.29 to 10.43 log10 GC l-1, with a median of 9.46 ± 0.40 log10 GC l-1, presenting both spatial and temporal variabilities.

Conclusions: Although SARS-CoV-2 data normalization employing crAssphage revealed a correlation with clinical cases occurring during the study period, crAssphage normalization by the flow per capita per day of each WWTP increased this correlation, corroborating the importance of normalizing wastewater surveillance data in disease trend monitoring.

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将 crAssphage 作为生物变量进行评估，以便在废水监测中对 SARS-CoV-2 数据进行归一化处理。

目的：本研究旨在通过多元归一化分析评估交叉组装噬菌体（crAssphage）作为内源性对照的使用及其作为 SARS-CoV-2 数据归一化的应用：在为期 1 年的监测期间，从 8 个污水处理厂（WWTP）共采集了 188 份 12 小时的原污水复合样本。利用 N1 和 N2 目标区，分别在 94% （177 份）和 90% （170 份）的样本中检测到 SARS-CoV-2 RNA，全球中位数为每升 5 log10 基因组拷贝（GC l-1）。100%的样本中都检测到了 CrAssphage，其范围为 8.29 至 10.43 log10 GC l-1，中位数为 9.46 ± 0.40 log10 GC l-1，具有空间和时间变异性：尽管采用crAssphage对SARS-CoV-2数据进行归一化处理后发现，该数据与研究期间出现的临床病例有一定的相关性，但根据每个污水处理厂的人均日流量对crAssphage进行归一化处理后，这种相关性有所提高，这也证实了在疾病趋势监测中对污水监测（WWS）数据进行归一化处理的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.