Thyroid dysfunction in Hashimoto's thyroiditis: a pilot study on the putative role of miR-29a and TGFβ1.

IF 3.7 3区医学 Q2 Medicine Endocrine Pub Date : 2024-12-01 Epub Date: 2024-07-17 DOI:10.1007/s12020-024-03965-3

Maria Consiglia Trotta, Daniela Esposito, Raffaela Carotenuto, Rosa di Fraia, Lucia Digitale Selvaggio, Francesca Allosso, Marina Russo, Giacomo Accardo, Roberto Alfano, Michele D'Amico, Daniela Pasquali

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Abstract

Purpose: Hashimoto's thyroiditis (HT) is one of the most common causes of thyroid dysfunction in iodine sufficient worldwide areas, but its molecular mechanisms are not completely understood. To this regard, this study aimed to assess serum levels of miRNA-29a (miR-29a) and transforming growth factor beta 1 (TGFβ1) in HT patients with different patterns of thyroid function.

Methods: A total of 29 HT patients, with a median age of 52 years (21-68) were included. Of these, 13 had normal thyroid function (Eu-HT); 8 had non-treated hypothyroidism (Hypo-HT); 8 had hypothyroidism on replacement therapy with LT4 (subst-HT). All patients had serum miR-29a assayed through qRT-PCR and serum TGFβ1 assayed by ELISA.

Results: Serum miR-29a levels were significantly down-regulated in patients with Hypo-HT compared to Eu-HT patients (P < 0.01) and subst-HT patients (P < 0.05). A significant negative correlation was detected between serum miR-29a levels and TSH levels (r = -0.60, P < 0.01). Serum TGFβ1 levels were significantly higher in Hypo-HT than both Eu-HT (P < 0.01) and subst-HT patients (P < 0.05). A negative correlation was observed between serum miR-29a and TGFβ1 (r = -0.75, P < 0.01).

Conclusions: In conclusion, Hypo-HT patients had lower levels of serum miR-29a and higher levels of TGFβ1 in comparison with Eu-HT patients. Worthy of note, subst-HT patients showed restored serum miR-29a levels compared with Hypo-HT group, associated with lower serum TGFβ1. These novel findings may suggest a possible impact of replacement therapy with levothyroxine on serum miR-29a levels in HT.

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桥本氏甲状腺炎患者的甲状腺功能障碍：一项关于 miR-29a 和 TGFβ1 推测作用的试验性研究。

目的：桥本氏甲状腺炎（HT）是全球碘充足地区甲状腺功能障碍最常见的原因之一，但其分子机制尚未完全明了。为此，本研究旨在评估甲状腺功能不同的桥本氏甲状腺炎患者血清中 miRNA-29a (miR-29a) 和转化生长因子β1 (TGFβ1) 的水平：共纳入29名甲状腺肿大患者，中位年龄为52岁（21-68岁）。其中 13 人甲状腺功能正常（Eu-HT）；8 人甲状腺功能减退未经治疗（Hypo-HT）；8 人甲状腺功能减退接受 LT4 替代治疗（subst-HT）。所有患者的血清 miR-29a 均通过 qRT-PCR 检测，血清 TGFβ1 则通过 ELISA 检测：结果：与 Eu-HT 患者相比，Hypo-HT 患者的血清 miR-29a 水平明显下调（P 结论：Hypo-HT 患者的血清 miR-29a 水平与 Eu-HT 患者相比明显下降（P）：总之，与 Eu-HT 患者相比，Hypo-HT 患者血清 miR-29a 水平较低，TGFβ1 水平较高。值得注意的是，与低HT组相比，亚HT组患者的血清miR-29a水平有所恢复，与较低的血清TGFβ1水平相关。这些新发现表明，左甲状腺素替代疗法可能会对高密度脂蛋白血症患者的血清 miR-29a 水平产生影响。

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来源期刊

Endocrine 医学-内分泌学与代谢

CiteScore

6.40

自引率

5.40%

发文量

期刊介绍： Well-established as a major journal in today’s rapidly advancing experimental and clinical research areas, Endocrine publishes original articles devoted to basic (including molecular, cellular and physiological studies), translational and clinical research in all the different fields of endocrinology and metabolism. Articles will be accepted based on peer-reviews, priority, and editorial decision. Invited reviews, mini-reviews and viewpoints on relevant pathophysiological and clinical topics, as well as Editorials on articles appearing in the Journal, are published. Unsolicited Editorials will be evaluated by the editorial team. Outcomes of scientific meetings, as well as guidelines and position statements, may be submitted. The Journal also considers special feature articles in the field of endocrine genetics and epigenetics, as well as articles devoted to novel methods and techniques in endocrinology. Endocrine covers controversial, clinical endocrine issues. Meta-analyses on endocrine and metabolic topics are also accepted. Descriptions of single clinical cases and/or small patients studies are not published unless of exceptional interest. However, reports of novel imaging studies and endocrine side effects in single patients may be considered. Research letters and letters to the editor related or unrelated to recently published articles can be submitted. Endocrine covers leading topics in endocrinology such as neuroendocrinology, pituitary and hypothalamic peptides, thyroid physiological and clinical aspects, bone and mineral metabolism and osteoporosis, obesity, lipid and energy metabolism and food intake control, insulin, Type 1 and Type 2 diabetes, hormones of male and female reproduction, adrenal diseases pediatric and geriatric endocrinology, endocrine hypertension and endocrine oncology.