Pseudomonas aeruginosa epidemic high-risk clones and their association with multidrug-resistant

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES Journal of global antimicrobial resistance Pub Date : 2024-07-15 DOI:10.1016/j.jgar.2024.07.003
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Abstract

Objective

In Ecuador, data on molecular epidemiology, as well as circulating clones, are limited. Therefore, this study aims to know the population structure of Pseudomonas aeruginosa by identifying clones in clinical samples in Quito-Ecuador.

Methods

A significant set (45) clinical P. aeruginosa isolates were selected, including multidrug and non-multidrug resistant isolates, which were assigned to sequence types (STs) and compared with their antibiotic susceptibility profile. The genetic diversity was assessed by applying the multilocus sequence typing (MLST) scheme and the genetic relationships between different STs were corroborated by phylogenetic networks.

Results

The MLST analysis identified 24 different STs and the most prevalent STs were ST-3750 and ST-253. The majority of the multidrug-resistance (MDR) isolates were included in ST-3750 and ST-253, also 3 singleton STs were identified as MDR isolates. The 21 different STs were found in non-multidrug resistance (non-MDR) isolates, and only 3 STs were found in more the one isolate.

Conclusions

The population structure of clinical P. aeruginosa present in these isolates indicates a significant association between MDR isolates and the clonal types: all ST-3750 and ST-253 isolates were MDR. ST-3750 is a closely related strain to the clonal complex ST111 (CC111). ST-253 and ST111 are a group of successful high-risk clones widely distributed worldwide. The multiresistant isolates studied are grouped in the most prevalent STs found, and the susceptible isolates correspond mainly with singleton STs. Therefore, these high-risk clones and their association with MDR phenotypes are contributing to the spread of MDR in Quito, Ecuador.

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铜绿假单胞菌流行病高危克隆及其与耐多药菌的关系
背景:在厄瓜多尔,有关分子流行病学以及循环克隆的数据十分有限。因此,本研究旨在通过鉴定厄瓜多尔基多省临床样本中的克隆,了解铜绿假单胞菌的种群结构:方法:选取了大量临床铜绿假单胞菌分离株(45 株),包括耐多药和非耐多药分离株,将其归入序列类型(ST),并与其抗生素敏感性谱进行比较。应用多焦点序列分型(MLST)方案评估了遗传多样性,并通过系统发育网络证实了不同 ST 之间的遗传关系:结果:MLST分析确定了24种不同的ST,最常见的ST是ST-3750和ST-253。大多数耐多药(MDR)分离株都属于 ST-3750 和 ST-253,还有 3 个单株 ST 被鉴定为 MDR 分离株。在非多重耐药性(非 MDR)分离株中发现了 21 种不同的 ST,只有 3 种 ST 在多个分离株中发现:结论:这些分离物中存在的临床铜绿假单胞菌的种群结构表明,多重耐药分离物与克隆类型之间存在显著关联:所有 ST-3750 和 ST-253 分离物均为多重耐药分离物。ST-3750 是与克隆复合体 ST111(CC111)密切相关的菌株。ST-253 和 ST111 是一组成功的高风险克隆,广泛分布于世界各地。所研究的多重耐药分离株按发现的最普遍序列类型分组,而易感分离株主要对应单序列类型。因此,这些高风险克隆及其与多重耐药性表型的关联正在助长多重耐药性在厄瓜多尔基多的蔓延。
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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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