{"title":"Review of SNP assays for disaster victim identification: Cost, time, and performance information for decision-makers","authors":"Katherine Butler Gettings PhD, Andreas Tillmar PhD, Kimberly Sturk-Andreaggi PhD, Charla Marshall PhD","doi":"10.1111/1556-4029.15585","DOIUrl":null,"url":null,"abstract":"<p>In mass disaster events, forensic DNA laboratories may be called upon to quickly pivot their operations toward identifying bodies and reuniting remains with family members. Ideally, laboratories have considered this possibility in advance and have a plan in place. Compared with traditional short tandem repeat (STR) typing, single nucleotide polymorphisms (SNPs) may be better suited to these disaster victim identification (DVI) scenarios due to their small genomic target size, resulting in an improved success rate in degraded DNA samples. As the landscape of technology has shifted toward DNA sequencing, many forensic laboratories now have benchtop instruments available for massively parallel sequencing (MPS), facilitating this operational pivot from routine forensic STR casework to DVI SNP typing. Herein, we present the commercially available SNP sequencing assays amenable to DVI, we use data simulations to explore the potential for kinship prediction from SNP panels of varying sizes, and we give an example DVI scenario as context for presenting the matrix of considerations: kinship predictive potential, cost, and throughput of current SNP assay options. This information is intended to assist laboratories in choosing a SNP system for disaster preparedness.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"69 5","pages":"1546-1557"},"PeriodicalIF":1.5000,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1556-4029.15585","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of forensic sciences","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1556-4029.15585","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, LEGAL","Score":null,"Total":0}
引用次数: 0
Abstract
In mass disaster events, forensic DNA laboratories may be called upon to quickly pivot their operations toward identifying bodies and reuniting remains with family members. Ideally, laboratories have considered this possibility in advance and have a plan in place. Compared with traditional short tandem repeat (STR) typing, single nucleotide polymorphisms (SNPs) may be better suited to these disaster victim identification (DVI) scenarios due to their small genomic target size, resulting in an improved success rate in degraded DNA samples. As the landscape of technology has shifted toward DNA sequencing, many forensic laboratories now have benchtop instruments available for massively parallel sequencing (MPS), facilitating this operational pivot from routine forensic STR casework to DVI SNP typing. Herein, we present the commercially available SNP sequencing assays amenable to DVI, we use data simulations to explore the potential for kinship prediction from SNP panels of varying sizes, and we give an example DVI scenario as context for presenting the matrix of considerations: kinship predictive potential, cost, and throughput of current SNP assay options. This information is intended to assist laboratories in choosing a SNP system for disaster preparedness.
在大规模灾难事件中,法医 DNA 实验室可能会被要求迅速将其业务转向确认尸体身份和帮助遗体与家人团聚。理想的情况是,实验室已经提前考虑到这种可能性,并制定了相应的计划。与传统的短串联重复(STR)分型相比,单核苷酸多态性(SNPs)可能更适合这些灾难受害者身份识别(DVI)方案,因为它们的基因组目标大小较小,从而提高了降解 DNA 样本的成功率。随着DNA测序技术的发展,许多法医实验室现在都拥有了可用于大规模并行测序(MPS)的台式仪器,从而促进了从常规法医STR案件工作到DVI SNP分型的操作转移。在本文中,我们介绍了可用于 DVI 的商用 SNP 测序方法,利用数据模拟探讨了不同规模 SNP 面板的亲缘关系预测潜力,并举例说明了 DVI 情景,以此为背景介绍了考虑因素矩阵:亲缘关系预测潜力、成本和当前 SNP 测定选项的吞吐量。这些信息旨在帮助实验室选择用于备灾的 SNP 系统。
期刊介绍:
The Journal of Forensic Sciences (JFS) is the official publication of the American Academy of Forensic Sciences (AAFS). It is devoted to the publication of original investigations, observations, scholarly inquiries and reviews in various branches of the forensic sciences. These include anthropology, criminalistics, digital and multimedia sciences, engineering and applied sciences, pathology/biology, psychiatry and behavioral science, jurisprudence, odontology, questioned documents, and toxicology. Similar submissions dealing with forensic aspects of other sciences and the social sciences are also accepted, as are submissions dealing with scientifically sound emerging science disciplines. The content and/or views expressed in the JFS are not necessarily those of the AAFS, the JFS Editorial Board, the organizations with which authors are affiliated, or the publisher of JFS. All manuscript submissions are double-blind peer-reviewed.