Development and Validation of a Quantitative Analysis of Water-Soluble Vitamins Using High-Performance Thin-Layer Chromatography and Its Application to the Analysis of Nutraceuticals
K. M. Y. K. Sikdar, Md Khairul Islam, Tomislav Sostaric, L. Y. Lim, C. Locher
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Abstract
In this study, a high-performance thin-layer chromatography (HPTLC)-based method was developed for the quantification of five water-soluble vitamins, B2, B3, B6, B12, and C, using ethanol–water (70:30, v/v) as the mobile phase. The method was validated according to the International Conference on Harmonisation (ICH) guidelines and the limits of detection/limits of quantification were determined as 5.27/15.97 ng (Vitamin B2), 13.03/39.47 ng (Vitamin B3), 59.61/180.64 ng (Vitamin B6), 14.17/42.93 ng (Vitamin B12), and 119.27/361.42 ng (Vitamin C) per band, respectively. The usefulness of the validated method for quality control was demonstrated with the successful quantitative analysis of the five vitamins in a range of nutraceuticals.
期刊介绍：
ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications.
The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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